CLASTOGENIC INOSINE NUCLEOTIDE AS COMPONENTS OF THE CHROMOSOME BREAKAGE FACTOR IN SCLERODERMA PATIENTS
CLASTOGENIC INOSINE NUCLEOTIDE AS COMPONENTS OF THE CHROMOSOME BREAKAGE FACTOR IN SCLERODERMA PATIENTS
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DOI：
10.1016/0003-9861(90)90253-u
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发表时间：
1990-04-01
影响因子：
3.9
通讯作者：
EMERIT, I
中科院分区：
文献类型：
--
作者：
AUCLAIR, C;GOUYETTE, A;EMERIT, I
在本研究中，我们试图从进行性系统性硬化症（硬皮病）患者中鉴定致染色体断裂因子（CF）的化学性质。通过HPLC获得的致染色体断裂组分的计算机质谱分析，血浆超滤检测到与三磷酸肌苷和二磷酸肌苷（ITP和IDP）相当的分子峰。同时检测到IDP和ITP，以及非致染色体断裂组分和相应对照组分中这些峰的消失是支持这些观察结果的生物学相关性的论据。最重要的确认来自加入试验培养物培养基中的市售ITP和IDP的致染色体断裂作用。这些物质在细胞周期G0期暴露的淋巴细胞中诱导染色单体型损伤，超氧化物歧化酶预防这种损伤与CF的观察结果类似。
In the present study, we attempted to identify the chemical nature of the clastogenic factor (CF) from patients with progressive systemic sclerosis (scleroderma). Computerized mass spectrometry of clastogenic fractions obtained by HPLC of plasma ultra-filtrates detected molecular peaks comparible with inosine triphosphate and inosine diphosphate (ITP and IDP). The concomitant detection of IDP, together with ITP, and the absene of these peaks in nonclastogenic fractions and corresponding control fractions are arguments in favor of a biological relevance of these observations. The most important confirmation came from the clastogenic effect of commercial ITP and IDP added to the culture medium of the test cultures. The induction of chromatid type damage by these substances in lymphocytes exposed in the G0 phase of their cell cycle and the prevention of this damage by superoxide dismutase are analogous to the observations with CF.