Effects of EGTA on the calcium-activated afterhyperpolarization in hippocampal CA3 pyramidal cells.
Effects of EGTA on the calcium-activated afterhyperpolarization in hippocampal CA3 pyramidal cells.
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DOI：
10.1126/science.6777871
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发表时间：
1980-12
期刊：
影响因子：
56.9
通讯作者：
P. Schwartzkroin;Carl E. Stafstrom
中科院分区：
文献类型：
--
作者：
P. Schwartzkroin;Carl E. Stafstrom
用填充有0.2摩尔钾EGTA的电极在海马切片制备中进行细胞内记录。目的是研究钙离子在介导由细胞内电流脉冲诱发的突发后超极化中的作用，自发产生，或由致癫痫药阿曲西林诱导。在前两种情况下，后超极化被阻断，相关的电导增加减少，这表明EGTA在超极化过程中阻断了钙激活的钾电导。在第三种情况下，后超极化和相关的电导增加持续存在，暗示了超极化的另一种机制。? 2Ns 04053
Intracellular recordings in the hippocampal slice preparation were made with electrodes filled with 0.2 molar potassium EGTA. The purpose was to investigate the role of calcium in mediating afterhyperpolarizations following bursts evoked by intracellular current pulses, arising spontaneously, or induced by the epileptogenic agent pencillin. In the first two cases the afterhyperpolarization was blocked and the associated conductance increase was reduced, suggesting that EGTA was blocking the calcium-activated potassium conductance during the hyperpolarization. In the third case the afterhyperpolarization and associated conductance increase persisted, implicating an alternative mechanism for the hyperpolarization. ?2Ns 04053