Isolation of Prasinoviruses of the Green Unicellular Algae Ostreococcus spp. on a Worldwide Geographical Scale
Isolation of Prasinoviruses of the Green Unicellular Algae Ostreococcus spp. on a Worldwide Geographical Scale
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DOI：
10.1128/aem.01799-09
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发表时间：
2010-01-01
影响因子：
4.4
通讯作者：
Desdevises, Yves
中科院分区：
文献类型：
--
作者：
Bellec, Laure;Grimsley, Nigel;Desdevises, Yves
牡蛎属是世界范围内海洋环境中发现的非常小的单细胞真核绿色藻类，它们容易受到各种大型DNA病毒的攻击。几种生物学上不同的Ostreococcus种类是已知的，它们所占据的生态位不同：而O。tauri（代表进化枝C菌株）发现于海洋泻湖和沿海海域，菌株属于进化枝A，例如O. Lucimarinus，存在于不同的海洋中。我们使用这两个物种的克隆分离株的实验室培养物来测定来自不同地点的海水样品中病毒的存在。与宿主菌的分布相一致，O.在法国西南部的一个泻湖中，相对于地中海，没有O.检测到金牛病毒。相反，感染O.在遥远的分离海洋中发现了Lucimarinus。DNA测序，系统发育分析使用一个保守的病毒标记基因，和一个Mantel测试显示没有关系的地理和系统发育距离之间的病毒感染O。露西马里纳斯。
Ostreococcus spp. are extremely small unicellular eukaryotic green algae found worldwide in marine environments, and they are susceptible to attacks by a diverse group of large DNA viruses. Several biologically distinct species of Ostreococcus are known and differ in the ecological niches that they occupy: while O. tauri (representing clade C strains) is found in marine lagoons and coastal seas, strains belonging to clade A, exemplified by O. lucimarinus, are present in different oceans. We used laboratory cultures of clonal isolates of these two species to assay for the presence of viruses in seawater samples from diverse locations. In keeping with the distributions of their host strains, we found a decline in the abundance of O. tauri viruses from a lagoon in southwest France relative to the Mediterranean Sea, whereas in the ocean, no O. tauri viruses were detected. In contrast, viruses infecting O. lucimarinus were detected from distantly separated oceans. DNA sequencing, phylogenetic analyses using a conserved viral marker gene, and a Mantel test revealed no relationship between geographic and phylogenetic distances in viruses infecting O. lucimarinus.