Correlation between biochemical composition and magnetic resonance appearance of articular cartilage
Correlation between biochemical composition and magnetic resonance appearance of articular cartilage
复制标题
DOI：
10.1053/joca.1997.0089
复制
发表时间：
1998-01-01
影响因子：
7
通讯作者：
Vittur, F
中科院分区：
文献类型：
--
作者：
Fragonas, E;Mlynárik, V;Vittur, F
目的：本研究的目的是找到磁共振（MR）的外观和生物化学组成的正常关节软骨的两个主要基质成分的分布比较层状方面：蛋白多糖和collagen.Design：T-2加权MR显微图像的猪软骨骨栓，切除的习惯加载和习惯卸载的肱骨近端的区域之间的相关性。使用自旋回波序列获得。蛋白聚糖（PG）进行了监测组织学和测量的糖醛酸和硫含量的组织;组织学方法和羟脯氨酸的化学测定用于评价的胶原contents.Results：“加载”软骨表现出预期的MR层状外观，而“卸载”组织似乎更均匀。PG含量在“加载”软骨，被发现是2.4倍，高于习惯性卸载组织，表现出增加的趋势，从关节面到骨。在“卸载”软骨中，糖醛酸分布更均匀，中间区浓度更高。发现两个软骨区域的平均胶原含量为组织干重的约39%。组织学和羟脯氨酸的分布模式表明，胶原蛋白是特别集中在表面和“加载”软骨的中心区域，而在“卸载”组织胶原蛋白是明显的，只在表面。根据胶原蛋白的分布，横向松弛（T-2）时间在“加载”的软骨表现出一个最小值在关节面和另一个最小值在中心区域。相反，平均Tr值的“卸载”组织是高的表面和迅速下降，在较深的zones.Conclusion：这些结果表明，关节软骨的MR外观与胶原含量，但不与PG，不同的区域。然而，其他基质成分可能通过促进组织的大分子组织而影响MR外观。
Objective: The objective of this study was to find a correlation between magnetic resonance (MR) appearance and biochemical composition of the normal articular cartilage by comparing the laminar aspects with the distribution of the two principal matrix components: proteoglycans and collagen.Design: T-2-weighted MR microimages of porcine cartilage-bone plugs, excised from both the habitually loaded and habitually unloaded regions of the proximal end of the humerus. were obtained using a spin-echo sequence. Proteoglycans (PGs) were monitored by histology and by measuring the uronate and the sulfur content of the tissue; a histologic method and the chemical determination of hydroxyproline were used for the evaluation of the collagen content.Results: The 'loaded' cartilage exhibited the expected MR laminar appearance whereas the 'unloaded' tissue appeared to be more homogeneous. The PG content in the 'loaded' cartilage, was found to be 2.4 times higher than in the habitually unloaded tissue, exhibiting an increasing trend from the articular surface to the bone. In the 'unloaded' cartilage the uronate distribution was more uniform with a higher concentration in the intermediate zone. The mean collagen content of both cartilage regions was found to be about 39% of the tissue dry weight. Histology and hydroxyproline distribution pattern showed that collagen was particularly concentrated at the surface and in a central zone of the 'loaded' cartilage whereas in the 'unloaded' tissue collagen was evident only at the surface. In accordance with the collagen distribution, transverse relaxation (T-2) times in 'loaded' cartilage showed a minimum value at the articular surface and another minimum in a central region. On the contrary, the average Tr value of the 'unloaded' tissue was high at the surface and decreased rapidly in the deeper zones.Conclusion: These results demonstrate that the MR appearance of articular cartilage correlates with the collagen content, but not with that of PGs, of the different zones. Other matrix components might, however, influence the MR appearance by contributing to the macromolecular organization of the tissue.