Differentiation of neuroblastoma cell line N1E-115 involves several signaling cascades
Differentiation of neuroblastoma cell line N1E-115 involves several signaling cascades
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DOI：
10.1007/s11064-005-2607-2
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发表时间：
2005-03-01
影响因子：
4.4
通讯作者：
Lubec, G
中科院分区：
文献类型：
--
作者：
Oh, J;Karlmark, K;Lubec, G
尚未发表对未分化细胞谱系与分化细胞谱系中信号蛋白 (SP) 差异表达的系统搜索，本文中我们为此目的使用蛋白质分析。培养 N1E-115 细胞系，并用二甲亚砜 (DMSO) 分化等分试样，已知这会导致神经元表型。制备细胞裂解物，进行二维凝胶电泳，然后对蛋白质进行 MALDI-TOF-TOF 鉴定，并生成所鉴定的 SP 的图谱。 7 个 SP 具有可比性，27 个 SP：GTP 结合/Ras 相关蛋白、激酶、生长因子、钙结合蛋白、磷酸酶相关蛋白在分化的 N1E-115 细胞中观察到，上述组中的 8 个 SP 仅在未分化细胞中观察到。在分化过程中，来自不同信号级联的多个单独的 SP 的打开/关闭是理解所涉及机制的关键。本文报道的发现对于证实紊乱的 SP 的功能作用的体外和体内研究具有挑战性。
No systematic searches for differential expression of signaling proteins (SP) in undifferentiated vs. differentiated cell lineages were published and herein we used protein profiling for this purpose. The N1E-115 cell line was cultivated and an aliquot was differentiated with dimethylsulfoxide (DMSO), that is known to lead to a neuronal phenotype. Cell lysates were prepared, run on two-dimensional gel electrophoresis followed by MALDI-TOF-TOF identification of proteins and maps of identified SPs were generated. Seven SPs were comparable, 27 SPs: GTP-binding/Ras-related proteins, kinases, growth factors, calcium binding proteins, phosphatase-related proteins were observed in differentiated N1E-115 cells and eight SPs of the groups mentioned above were observed in undifferentiated cells only. Switching-on/off of several individual SPs from different signaling cascades during the differentiation process is a key to understand mechanisms involved. The findings reported herein are challenging in vitro and in vivo studies to confirm a functional role for deranged SPs.