Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila
Sequential pulses of apical epithelial secretion and endocytosis drive airway maturation in Drosophila
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DOI：
10.1016/j.devcel.2007.06.008
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发表时间：
2007-08-01
影响因子：
11.8
通讯作者：
Samakovlis, Christos
中科院分区：
文献类型：
--
作者：
Tsarouhas, Vasilios;Senti, Kirsten-André;Samakovlis, Christos
充满空气的呼吸器官的发育对出生时的生存至关重要。我们结合活体成像和遗传分析来解剖果蝇胚胎气管中呼吸器官的成熟过程。我们发现气管导管的成熟需要三个精确的上皮转变。最初，分泌物爆发将蛋白质沉积到管腔中。然后从管中快速清除固体管腔材料，此后不久除去液体。为了阐明这些转变背后的细胞机制，我们确定了气体填充缺陷突变体显示狭窄或蛋白质堵塞管。这些突变要么破坏内质网到高尔基体囊泡运输或包合。首先，Sar1是蛋白质分泌、管腔基质组装和直径管扩张所必需的。随后，Rab5依赖性内吞活性的尖锐脉冲迅速内化并清除管腔内容物。管腔基质分泌和内吞的协调可能是管状器官形态发生和成熟的一般机制。
The development of air-filled respiratory organs is crucial for survival at birth. We used a combination of live imaging and genetic analysis to dissect respiratory organ maturation in the embryonic Drosophila trachea. We found that tracheal tube maturation entails three precise epithelial transitions. Initially, a secretion burst deposits proteins into the lumen. Solid luminal material is then rapidly cleared from the tubes, and shortly thereafter liquid is removed. To elucidate the cellular mechanisms behind these transitions, we identified gas-filling-deficient mutants showing narrow or protein-clogged tubes. These mutations either disrupt endoplasmatic reticulum-to-Golgi vesicle transport or enclocytosis. First, Sar1 is required for protein secretion, luminal matrix assembly, and diametric tube expansion. Subsequently, a sharp pulse of Rab5-dependent endocytic activity rapidly internalizes and clears luminal contents. The coordination of luminal matrix secretion and endocytosis may be a general mechanism in tubular organ morphogenesis and maturation.