The serum response factor coactivator myocardin is required for vascular smooth muscle development
The serum response factor coactivator myocardin is required for vascular smooth muscle development
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DOI：
10.1073/pnas.1233635100
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发表时间：
2003-08-05
影响因子：
11.1
通讯作者：
Olson, EN
中科院分区：
文献类型：
--
作者：
Li, SJ;Wang, DZ;Olson, EN
血管系统的形成需要内皮细胞和平滑肌细胞（SMC）的分化和图案化。虽然很多注意力都集中在血管内皮网络的发展，控制血管SMC发展的机制在很大程度上是未知的。肌心素是一种平滑肌特异性血清反应因子的转录共激活因子，是一种普遍存在的涉及平滑肌基因表达的转录因子。当在非肌细胞异位表达时，myocardin可通过与血清反应因子的结合诱导平滑肌分化。在这里，我们报告说，小鼠胚胎myocardin功能丧失突变纯合子死亡的胚胎10.5天，没有证据表明血管平滑肌细胞分化。肌心蛋白是已知的唯一的转录因子是必要的和足够的血管平滑肌细胞分化。
Formation of the vascular system requires differentiation and patterning of endothelial and smooth muscle cells (SMCs). Although much attention has focused on development of the vascular endothelial network, the mechanisms that control vascular SMC development are largely unknown. Myocardin is a smooth and cardiac muscle-specific transcriptional coactivator of serum response factor, a ubiquitous transcription factor implicated in smooth muscle gene expression. When expressed ectopically in nonmuscle cells, myocardin can induce smooth muscle differentiation by its association with serum response factor. Here we report that mouse embryos homozygous for a myocardin loss-of-function mutation die by embryonic day 10.5 and show no evidence of vascular SMC differentiation. Myocardin is the only transcription factor known to be necessary and sufficient for vascular SMC differentiation.