Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo - a preliminary study.
Darkfield orthogonal polarized spectral imaging for studying endovascular laser-tissue interactions in vivo - a preliminary study.
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DOI：
10.1364/opex.13.000702
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发表时间：
2005-02
期刊：
影响因子：
3.8
通讯作者：
M. Heger;J. Beek;Karin Stenback;D. Faber;M. V. van Gemert;C. Ince
中科院分区：
文献类型：
--
作者：
M. Heger;J. Beek;Karin Stenback;D. Faber;M. V. van Gemert;C. Ince
由于合适的活体显微镜技术的数量有限，对选择性光热疗法的光热（内）血管反应知之甚少，选择性光热疗法用作浅表血管异常（如葡萄酒色斑、毛细血管扩张和血管瘤）的默认治疗方式。在这项初步研究中，我们提出了一种新的显微镜技术研究（内）血管激光组织在体内的相互作用，在传统的正交偏振光谱（OPS）成像相结合的暗场（DF）照明。DFOPS成像的大鼠肠系膜血管照射功率增加，揭示了以下（组织）的反应：形成半透明的聚集体，逆行流动，逐渐和立即止血，恢复流动，血管消失，血管周围胶原蛋白损伤。因此，DFOPS成像构成了一个有用的工具，检查（内）血管事件后选择性光疗。
Due to the limited number of suitable intravital microscopy techniques, relatively little is known about the opto-thermal (endo)vascular responses to selective photothermolysis, used as a default treatment modality for superficial vascular anomalies such as port wine stains, telangiectasias, and hemangiomas. In this preliminary study we present a novel microscopy technique for studying (endo)vascular laser-tissue interactions in vivo, in which conventional orthogonal polarized spectral (OPS) imaging is combined with darkfield (DF) illumination. DFOPS imaging of rat mesenteric vasculature irradiated at increasing powers revealed the following (tissular) responses: formation of translucent aggregates, retrograde flow, gradual and immediate hemostasis, reinstatement of flow, vessel disappearance, and perivascular collagen damage. DFOPS imaging therefore constitutes a useful tool for examining (endo)vascular events following selective photothermolysis.