SLIDING MOVEMENT OF SINGLE ACTIN-FILAMENTS ON ONE-HEADED MYOSIN-FILAMENTS
SLIDING MOVEMENT OF SINGLE ACTIN-FILAMENTS ON ONE-HEADED MYOSIN-FILAMENTS
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DOI：
10.1038/326805a0
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发表时间：
1987-04-23
期刊：
影响因子：
64.8
通讯作者：
YANAGIDA, T
中科院分区：
文献类型：
--
作者：
HARADA, Y;NOGUCHI, A;YANAGIDA, T
肌球蛋白分子由两个头部组成，每个头部含有一个酶活性位点和一个肌动蛋白结合位点。在肌肉收缩过程中，两个头部是独立还是协同作用的基本问题已经通过使用肌动球蛋白线1，超沉淀2 - 4和肌肉组织化学修饰5的方法进行了研究。目前还没有明确的结论。我们已经使用一种测定系统来处理这个问题，在该系统中，可以直接观察到荧光标记的单个肌动蛋白丝沿着肌球蛋白丝的滑动运动6，7。在这里，我们报告的滑动单肌动蛋白丝沿着一个头的肌球蛋白丝，其中头的密度在很大范围内变化的直接测量。我们的研究结果表明，肌球蛋白的两个头部之间的合作相互作用是不是必不可少的诱导肌动蛋白丝的滑动运动。
The myosin molecule consists of two heads, each of which contains an enzymatic active site and an actin-binding site. The fundamental problem of whether the two heads function independently or cooperatively during muscle contraction has been studied by methods using an actomyosin thread1, superprecipitation2–4and chemical modification of muscle fibres5. No clear conclusion has yet been reached. We have approached this question using an assay system in which sliding movements of fluorescently labelled single actin filaments along myosin filaments can be observed directly6,7. Here, we report direct measurement of the sliding of single actin filaments along one-headed myosin filaments in which the density of heads was varied over a wide range. Our results show that cooperative interaction between the two heads of myosin is not essential for inducing the sliding movement of actin filaments.