Oxidative stress responses in older men during endurance training and detraining
Oxidative stress responses in older men during endurance training and detraining
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DOI：
10.1249/01.mss.0000147632.17450.ff
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发表时间：
2004-12-01
期刊：
影响因子：
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通讯作者：
Deliconstantinos, G
中科院分区：
文献类型：
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作者：
Fatouros, IG;Jamurtas, AZ;Deliconstantinos, G
目的：衰老与氧化应激增加有关，而系统的运动训练已被证明可以改善老年人的生活质量和功能表现。本研究旨在评估在耐力训练和去训练期间，不运动的老年男性氧化应激和抗氧化状态的选定标志物的反应。方法：19名老年男性（65-78岁）被随机分为对照组（C, N = 8）和耐力训练组（ET, N = 11，每周三次训练，16周，步行/慢跑，HRmax的50-80%）。在训练前、训练后和去训练4个月后，受试者进行渐进式诊断跑步机疲劳测试（GXT）。采集gxt前后的血浆样本，分析丙二醛（MDA）和3-硝基酪氨酸（3-NT）水平、总抗氧化能力（TAC）和谷胱甘肽过氧化物酶活性（GPX）。结果：ET使跑步时间增加40%，最大耗氧量（VO2max）增加20% （P < 0.05）。ET降低MDA（休息时9%，P < 0.01；运动后16%，P < 0.05）和3-NT（运动后20%，P < 0.05）水平，而提高TAC（休息时6%，P < 0.01；运动后14%，P < 0.05）和GPX（运动后12%，P < 0.05）水平。然而，去训练消除了这些适应。结论：ET可通过提高TAC和GPX活性，减轻基础性和运动性脂质过氧化，增强对氧化应激的保护作用。然而，停止训练可能会逆转这些训练引起的适应。
Purpose: Aging is associated with increased oxidative stress, whereas systematic exercise training has been shown to improve quality of life and functional performance of the aged. This study aimed to evaluate responses of selected markers of oxidative stress and antioxidant status in inactive older men during endurance training and detraining.Methods: Nineteen older men (65-78 yr) were randomly assigned into either a control (C, N = 8) or an endurance-training (ET, N = 11, three training sessions per week, 16 wk, walking/jogging at 50-80% of HRmax) group. Before, immediately posttraining, and after 4 months of detraining, subjects performed a progressive diagnostic treadmill test to exhaustion (GXT). Plasma samples, collected before and immediately post-GXT, were analyzed for malondialdehyde (MDA) and 3-nitrotyrosine (3-NT) levels, total antioxidant capacity (TAC), and glutathione peroxidase activity (GPX).Results: ET caused a 40% increase in running time and a 20% increase in maximal oxygen consumption (VO2max) (P < 0.05). ET lowered MDA (9% at rest, P < 0.01; and 16% postexercise, P < 0.05) and 3-NT levels (20% postexercise, P < 0.05), whereas it increased TAC (6% at rest, P < 0.01; and 14% postexercise, P < 0.05) and GPX (12% postexercise, P < 0.05). However, detraining abolished these adaptations.Conclusions: ET may attenuate basal and exercise-induced lipid peroxidation and increase protection against oxidative stress by increasing TAC and GPX activity. However, training cessation may reverse these training-induced adaptations.