DIFFERENTIAL EXPRESSION OF IMMEDIATE EARLY GENES AFTER HIPPOCAMPAL LONG-TERM POTENTIATION IN AWAKE RATS
DIFFERENTIAL EXPRESSION OF IMMEDIATE EARLY GENES AFTER HIPPOCAMPAL LONG-TERM POTENTIATION IN AWAKE RATS
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DOI：
10.1016/0169-328x(93)90012-e
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发表时间：
1993-03-01
期刊：
影响因子：
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通讯作者：
TATE, WP
中科院分区：
文献类型：
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作者：
DEMMER, J;DRAGUNOW, M;TATE, WP
在清醒大鼠的齿状回中研究了长时程增强（LTP）诱导后fos和jun家族即早基因的表达模式。在LTP诱导的穿通通路强直化后，齿状回c-jun和jun-B mRNA和蛋白水平以及Fos相关蛋白（FRA）水平出现快速、短暂的增加，而jun-D mRNA和蛋白的诱导出现延迟和更长时间。通过施用N-甲基-D-天冬氨酸受体拮抗剂（其也阻断LTP诱导）和戊巴比妥（其减少但不阻断LTP）来防止c-Jun、Jun-B、Jun-D和Fos相关蛋白的诱导。这些发现表明，Fos和Jun基因家族成员的差异表达发生在清醒大鼠LTP后的一个独特的模式。反应基因可能参与导致突触修饰长期稳定的生化级联反应。
The pattern of expression of fos and jun family immediate early genes following the induction of long-term potentiation (LTP) was investigated in the dentate gyrus of awake rats. Rapid, transient increases in the levels of c-jun and jun-B mRNA and protein, and in the levels of Fos-related proteins (FRAs), occurred in the dentate gyrus after LTP-inducing tetanization of the perforant path. A delayed, and more prolonged induction occurred for jun-D mRNA and protein. The induction of c-Jun, Jun-B, Jun-D and Fos-related proteins was prevented by administration of an N-methyl-D-aspartate receptor antagonist, which also blocked LTP induction, and by pentobarbital, which reduced but did not block LTP. These findings show that differential expression of fos and jun gene family members occurs in a distinct pattern following LTP in awake rats. The responsive genes may participate in the biochemical cascade leading to the long-term stabilization of synaptic modifications.