Non-bronchoscopic sampling and culture of bronchial epithelial cells in children
Non-bronchoscopic sampling and culture of bronchial epithelial cells in children
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DOI：
10.1046/j.1365-2222.2003.01752.x
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发表时间：
2003-09-01
影响因子：
6.1
通讯作者：
Heaney, LG
中科院分区：
文献类型：
--
作者：
Doherty, GM;Christie, SN;Heaney, LG
背景支气管上皮细胞在儿童哮喘和病毒相关性喘息等气道疾病中起着重要作用。在成人中，对从纤维支气管镜获得的样本中培养的原代支气管上皮细胞的研究为上皮细胞的作用提供了关键的见解。然而，这是很难证明支气管镜在儿童中获得上皮细胞的research purpose.Objective检索和培养可行的上皮细胞使用一个盲目的非支气管镜方法从儿童择期surgery.Methods科目的可能性进行择期手术的儿童全身麻醉下。插管后，进行非支气管镜支气管肺泡灌洗和非支气管镜支气管刷拭。将带护套的支气管细胞学刷推进通过气管内导管，楔入，然后撤回2-3 cm，然后使用温和取样来收集支气管上皮细胞。与接受标准支气管镜采样的成人对照组相比，使用初始样本表征回收的上皮细胞的数量、类型和活力。随后的样本被用来建立原代支气管上皮细胞培养的儿童有和没有喘息性疾病。结果共63名儿童进行支气管刷[38名男性，中位年龄7.1岁（1.0-14.2岁）。初始样本（n = 30）显示，活上皮细胞的回收率与成人对照组（n = 11）中通过支气管镜获得的单个刷的回收率相当。在随后的33份非支气管镜儿童样本中，有27份（82%）成功建立了原代支气管上皮细胞培养。有没有不良影响归因于sampling.Conclusion我们已经表明，非支气管镜支气管刷是一种安全有效的技术，恢复可行的支气管上皮细胞，始终产生原代培养。这种方法将有助于检查上皮细胞在儿科疾病中的作用。
Background The bronchial epithelium is likely to play a vital role in airway diseases in children, such as asthma and viral-associated wheeze. In adults, studies with primary bronchial epithelial cells cultured from samples obtained by fibre-optic bronchoscopy have provided key insights into the role of the epithelial cell. However, it is difficult to justify bronchoscopy in children to obtain epithelial cells for research purposes.Objective To examine the possibility of retrieving and culturing viable epithelial cells using a blind non-bronchoscopic method from children undergoing elective surgery.Methods Subjects were children undergoing elective surgery under general anaesthesia. Following intubation, non-bronchoscopic bronchoalveolar lavage and non-bronchoscopic bronchial brushing were performed. A sheathed bronchial cytology brush was advanced through the endotracheal tube, wedged and then withdrawn 2-3 cm before gentle sampling was used to collect bronchial epithelial cells. Initial samples were used to characterize the number, type and viability of epithelial cells recovered compared to a control group of adults undergoing standard bronchoscopic sampling. Subsequent samples were used to establish primary bronchial epithelial cell cultures in children both with and without wheezing illness.Results A total of 63 children underwent bronchial brushing [38 male; median age 7.1 years (1.0-14.2 years]. Initial samples (n = 30) showed recovery of viable epithelial cells comparable to that from a single brush obtained via a bronchoscope in an adult control group (n = 11). In 27 (82%) of the subsequent 33 samples obtained non-bronchoscopically from children, primary bronchial epithelial cell cultures were successfully established. There were no adverse effects attributable to sampling.Conclusion We have shown that non-bronchoscopic bronchial brushing is a safe and effective technique for recovering viable bronchial epithelial cells that consistently yield primary cultures. This method will facilitate examination of the role of the epithelium in paediatric disease.