Cultured cerebellar cells as an in vitro model of excitatory amino acid receptor function.
Cultured cerebellar cells as an in vitro model of excitatory amino acid receptor function.
复制标题
培养的小脑细胞作为兴奋性氨基酸受体功能的体外模型。
DOI：
10.1016/0006-8993(87)90469-0
复制
发表时间：
1987
期刊：
影响因子：
2.9
通讯作者：
Morgan,WW
中科院分区：
文献类型：
--
作者：
McCaslin,PP;Morgan,WW
将8天大大鼠幼崽的神经元原代培养物在无抗生素培养基中体外培养10天，然后分析环鸟苷一磷酸（cGMP）对几种兴奋性氨基酸（EAA）激动剂或相关拮抗剂的反应变化。红藻氨酸（KA），N-甲基-d-天冬氨酸（NMDA）和使君子酸（QA）产生剂量和钙依赖性增加cGMP与KA产生最大和QA最小的增加，在这个环核苷酸。NMDA诱导的增加与KA和QA都是加性的，但是KA和QA彼此不加性。事实上，QA完全拮抗KA的作用，并且比EAA拮抗剂谷氨酰氨基甲磺酸（GAMS）或顺式-2，3-哌啶二羧酸（PDA）的拮抗程度大得多。2-氨基-7-膦酰基庚酸完全阻止NMDA诱导的cGMP升高，但对KA或QA诱导的该参数升高几乎没有影响。GAMS和PDA，另一方面，更有效地阻断KA和QA比NMDA的影响。这些数据表明，cGMP水平在小脑颗粒细胞提供了一个很好的模型，研究亚型的EAA受体。
Primary cultures of neurons from 8-day-old rat pups were grown for 10 days in vitro in antibiotic-free media and then analysed for changes in cyclic guanosine monophosphate (cGMP) in response to several excitatory amino acid (EAA) agonists or related antagonists. Kainic acid (KA), N-methyl-d-aspartic acid (NMDA) and quisqualic acid (QA) produced dose- and calcium-dependent increases in cGMP with KA producing the largest and QA the least increase in this cyclic nucleotide. The increase induced by NMDA was additive with both KA and QA; however, KA and QA were not additive with each other. In fact, QA completely antagonized the effects of KA and to a much greater degree than did the EAA antagonists, glutamylaminomethylsulfonic acid (GAMS) or cis-2,3-piperidine dicar☐ylic acid (PDA). 2-Amino-7-phosphonoheptanoic acid completely prevented the NMDA-induced elevations of cGMP yet had little effect on either the KA- or QA-induced elevations of this parameter. GAMS and PDA, on the other hand, were more effective in blocking the effects of KA and QA than of NMDA. These data show that cGMP levels in cerebellar granule cells provide an excellent model for studying the subtypes of EAA receptors.