Detection of latent cytomegalovirus in murine salivary and prostate explant cultures and cells
Detection of latent cytomegalovirus in murine salivary and prostate explant cultures and cells
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鼠唾液和前列腺外植体培养物和细胞中潜伏巨细胞病毒的检测
DOI：
10.1128/iai.15.2.568-574.1977
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发表时间：
1977
影响因子：
3.1
通讯作者：
D. Lang
中科院分区：
文献类型：
--
作者：
K. S. Cheung;D. Lang
感染成年小鼠后，通过组织培养接种长达 3 个月，在唾液腺悬浮液中可检测到巨细胞病毒。在这些培养物转为阴性后，可以在唾液腺和前列腺的外植体以及源自这些外植体的细胞系中检测到明显潜伏在这些组织中的病毒。在某些情况下，观察到病毒诱导的细胞损伤和再生的循环。在来自先前感染和未感染小鼠的前列腺和唾液腺细胞培养物中评估了鼠巨细胞病毒斑块效率和形态。尽管先前感染小鼠的前列腺细胞中的斑块大小发生了变化（更大），但没有检测到干扰的证据。这些研究表明，在活动性感染消退后，可检测到鼠巨细胞病毒的一系列抑制、潜伏或影响的存在，并为进一步研究病毒潜伏的建立和激活提供了方法。
After infection of adult mice, cytomegalovirus was detectable in salivary gland suspensions by tissue culture inoculation for up to 3 months. After these cultures had become negative, virus apparently latent in these tissues could be detected in explants of salivary and prostate glands and in cell lines derived from these explants. In some cases cycles of virus-induced cell injury and regrowth were observed. Murine cytomegalovirus plaque efficiency and morphology were evaluated in prostate and salivary gland cell cultures derived from previously infected and uninfected mice. No evidence of interference was detected, although plaques size was altered (larger) in prostate cells from previously infected mice. These studies indicate the presence of a range of suppression, latency, or effects of murine cytomegalovirus detectable after the resolution of active infection and provide methods for additional study of the establishment and activation of virus latency.