Wide field fluorescence epi-microscopy behind a scattering medium enabled by speckle correlations
Wide field fluorescence epi-microscopy behind a scattering medium enabled by speckle correlations
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DOI：
10.1364/oe.26.009866
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发表时间：
2018-04-16
期刊：
影响因子：
3.8
通讯作者：
Bertolotti, Jacopo
中科院分区：
文献类型：
--
作者：
Hofer, Matthias;Soeller, Christian;Bertolotti, Jacopo
荧光显微镜广泛用于生物成像，然而组织的散射强烈限制了其在浅深度的适用性。在这项工作中，我们采用了一种受恒星散斑干涉测量法启发的方法，并利用光学记忆效应使荧光显微镜能够透过浑浊层。我们展示了在落射显微镜中散射介质后面的微米尺寸荧光物体的有效重建，并研究了与传统显微镜相比这种成像方式（放大倍数、视场、分辨率）的特殊性。使用改进的相位检索算法从散斑图像重建荧光物体，即使在信噪比相对较低的条件下，我们也证明了稳健的重建。这种模式特别适合在生物介质中成像，已知生物介质表现出与数十微米大小的视场兼容的相对大的光学存储范围，以及与数十纳米宽度的发射荧光光谱兼容的大光谱带宽。由光学协会根据知识共享署名 4.0 许可条款发布。
Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and exploit the optical memory effect to enable fluorescence microscopy through a turbid layer. We demonstrate efficient reconstruction of micrometer-size fluorescent objects behind a scattering medium in epimicroscopy, and study the specificities of this imaging modality (magnification, field of view, resolution) as compared to traditional microscopy. Using a modified phase retrieval algorithm to reconstruct fluorescent objects from speckle images, we demonstrate robust reconstructions even in relatively low signal to noise conditions. This modality is particularly appropriate for imaging in biological media, which are known to exhibit relatively large optical memory ranges compatible with tens of micrometers size field of views, and large spectral bandwidths compatible with emission fluorescence spectra of tens of nanometers widths. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.