Immobilization and direct electrochemistry of glucose oxidase on a tetragonal pyramid-shaped porous ZnO nanostructure for a glucose biosensor
Immobilization and direct electrochemistry of glucose oxidase on a tetragonal pyramid-shaped porous ZnO nanostructure for a glucose biosensor
复制标题
用于葡萄糖生物传感器的四角锥状多孔 ZnO 纳米结构上葡萄糖氧化酶的固定化和直接电化学
DOI：
10.1016/j.bios.2008.07.047
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发表时间：
2009-01-01
影响因子：
12.6
通讯作者：
Shen, Jian
中科院分区：
文献类型：
--
作者：
Dai, Zhihui;Shao, Guojian;Shen, Jian
一种四方形多孔ZnO纳米结构（TPSP-ZnO）用于蛋白质的固定化、直接电化学和生物传感。所制备的ZnO具有较大的比表面积和良好的生物相容性。以葡萄糖氧化酶（GOD）为模型，研究了这种形貌的ZnO对蛋白质的固定作用，并构建了具有良好电化学性能的电化学生物传感器。用原子力显微镜、N-2吸附等温线和电化学方法研究了GOD与TPSP-ZnO的相互作用。固定化GOD在TPSP-ZnO修饰玻碳电极上表现出良好的直接电化学行为，这取决于TPSP-ZnO的性质。基于还原型GOD对溶解氧的电催化响应的降低，所提出的生物传感器在-0.50 V的施加电位下对葡萄糖浓度在0.05至8.2 mM范围内具有0.01 mM的检测限，具有比其他形态的ZnO纳米颗粒更好的生物传感特性。该生物传感器具有良好的稳定性、重现性和低干扰性，可快速、灵敏地诊断糖尿病。这种TPSP-ZnO纳米结构为蛋白质固定化和生物传感器的制备提供了良好的基质。(C)2008 Elsevier B. V.保留所有权利。
A tetragonal pyramid-shaped porous ZnO (TPSP-ZnO) nanostructure is used for the immobilization, direct electrochemistry and biosensing of proteins. The prepared ZnO has a large surface area and good biocompatibility. Using glucose oxidase (GOD) as a model, this shaped ZnO is tested for immobilization of proteins and the construction of electrochemical biosensors with good electrochemical performances. The interaction between GOD and TPSP-ZnO is examined by using AFM, N-2 adsorption isotherms and electrochemical methods. The immobilized GOD at a TPSP-ZnO-modified glassy carbon electrode shows a good direct electrochemical behavior, which depends on the properties of the TPSP-ZnO. Based on a decrease of the electrocatalytic response of the reduced form of GOD to dissolved oxygen, the proposed biosensor exhibits a linear response to glucose concentrations ranging from 0.05 to 8.2 mM with a detection limit of 0.01 mM at an applied potential of -0.50 V which has better biosensing properties than those from other morphological ZnO nanoparticles. The biosensor shows good stability, reproducibility, low interferences and can diagnose diabetes very fast and sensitively. Such the TPSP-ZnO nanostructure provides a good matrix for protein immobilization and biosensor preparation. (C) 2008 Elsevier B.V. All rights reserved.