Genome-Wide Expression Changes in Saccharomyces cerevisiae in Response to High-LET Ionizing Radiation
Genome-Wide Expression Changes in Saccharomyces cerevisiae in Response to High-LET Ionizing Radiation
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DOI：
10.1007/s12010-009-8825-3
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发表时间：
2010-10-01
影响因子：
3
通讯作者：
Hoshi, Masaharu
中科院分区：
文献类型：
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作者：
Mizukami-Murata, Satomi;Iwahashi, Hitoshi;Hoshi, Masaharu
为了了解酵母对高线性能量转移（LET）电离辐射（IR）的反应，我们使用DNA微阵列分析研究了三种类型的高LET IR（快中子，重离子和热中子）和伽马射线照射酵母的全局基因表达。静止的细胞照射每个IR和酵母蛋白胨葡萄糖培养基中重新培养，以允许修复40分钟。RNA，然后从三个独立的样品中分离出照射酵母。参与Mec 1 p激酶途径的基因在DNA损伤反应中起作用，被所有形式的高LET IR和γ射线诱导。一些与氧化应激和细胞壁相关的基因被各种形式的高LET IR诱导。通过单向方差分析对基因表达模式作为每种类型的高LET IR的函数进行统计学检查。这一分析表明存在辐射特异性反应。例如，参与核糖体DNA合成的基因被快中子辐射特异性地诱导，而泛素-蛋白酶体系统和热休克反应被热中子辐射特异性地诱导。该研究表征了高LET IR诱导的基因表达，并提供了对酵母后续适应的分子理解。
To understand the yeast response to high-linear energy transfer (LET) ionizing radiation (IR), we investigated global gene expression in yeast irradiated by three types of high-LET IR (fast neutrons, heavy ions, and thermal neutrons) and gamma rays using DNA microarray analysis. Stationary cells were irradiated by each IR and recultured in yeast-peptone-dextrose medium to allow repair for 40 min. RNA was then isolated from three independent samples of irradiated yeast. Genes involved in the Mec1p kinase pathway, which functions in DNA damage response, were induced by all forms of high-LET IR and by gamma rays. Some genes related to oxidative stress and the cell wall were induced by all forms of high-LET IRs. Gene expression patterns as a function of each type of high-LET IR were examined statistically by one-way analysis of variance. This analysis demonstrated the existence of irradiation-specific responses. For example, genes involved in ribosomal DNA synthesis were specifically induced by fast neutron irradiation, while the ubiquitin-proteasome system and heat shock response were specifically induced by thermal neutron irradiation. The study characterizes high-LET IR-induced gene expression and provides a molecular understanding of subsequent adaptation in yeast.