Stimulation of the ferroxidase activity of ceruloplasmin during iron loading into ferritin.
Stimulation of the ferroxidase activity of ceruloplasmin during iron loading into ferritin.
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在铁加载到铁蛋白期间刺激铜蓝蛋白的亚铁氧化酶活性。
DOI：
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发表时间：
1997
影响因子：
3.9
通讯作者：
S. Aust
中科院分区：
文献类型：
--
作者：
C. Reilly;S. Aust
从马血清中纯化的血浆铜蓝蛋白在加入增加当量的亚铁后迅速还原，产生电子和构象不同的形式。这种形式的血浆铜蓝蛋白的特征在于EPR可检测的I型和II型铜（II）的显著（80%）损失，在610 nm处的可见吸光度完全损失，以及疏水表面积减少。血浆铜蓝蛋白的还原形式缓慢地还原分子氧以完成其催化循环。不同浓度的脱铁铁蛋白，但不脱铁转铁蛋白的存在下，显着增强铜蓝蛋白氧化的速率。这种刺激作用的大小增加的铜蓝蛋白的摩尔比apoferitin接近1.0，以前被证明是最佳的比例加载铁到铁蛋白。铜蓝蛋白的亚铁氧化率显着刺激的存在下，脱铁铁蛋白，但是，脱铁转铁蛋白没有影响。血浆铜蓝蛋白氧化所有铁并返回酶的天然形式所需的时间长度也受铁浓度的影响。此外，铁负载到铁蛋白的速率取决于亚铁浓度。这些结果提供的证据之间的还原形式的血浆铜蓝蛋白和脱铁铁蛋白和还原的血浆铜蓝蛋白可能是复合物形成的信号之间的特定复合物的形成。
Ceruloplasmin purified from horse serum was rapidly reduced upon addition of increasing equivalents of ferrous iron, generating an electronically and conformationally distinct form. This form of ceruloplasmin was characterized by significant (80%) loss of EPR detectable type I and type II copper(II), complete loss of visible absorbance at 610 nm, as well as decreased hydrophobic surface area. The reduced form of ceruloplasmin slowly reduced molecular oxygen to complete its catalytic cycle. The presence of varied concentrations of apoferritin, but not apotransferrin, significantly enhanced the rate of ceruloplasmin oxidation. The magnitude of this stimulatory effect increased as the molar ratio of ceruloplasmin to apoferritin approached 1.0, shown previously to be the optimum ratio for loading iron into ferritin. The rate of ferrous iron oxidation by ceruloplasmin was significantly stimulated by the presence of apoferritin; however, apotransferrin had no effect. The length of time required for ceruloplasmin to oxidize all the iron and return to the native form of the enzyme was also affected by the concentration of iron. In addition, the rate of iron loading into ferritin was dependent upon ferrous iron concentration. These results provide evidence for the formation of a specific complex between the reduced form of ceruloplasmin and apoferritin and that reduction of ceruloplasmin by ferrous iron may be the signal for complex formation.
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影响因子：
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DOI：
--
发表时间：
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期刊：
The Journal of biological chemistry
影响因子：
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