Improved growth in a field trial of transgenic hybrid poplar overexpressing glutamine synthetase
Improved growth in a field trial of transgenic hybrid poplar overexpressing glutamine synthetase
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DOI：
10.1111/j.1469-8137.2004.01173.x
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发表时间：
2004-10-01
期刊：
影响因子：
9.4
通讯作者：
Cánovas, FM
中科院分区：
文献类型：
--
作者：
Jing, ZP;Gallardo, F;Cánovas, FM
在自然条件下，研究了表达一种谷氨酰胺合成酶（GS）基因的转基因杨树的生长性能。在西班牙格拉纳达省对8个独立的转基因株系和对照植株进行了为期3年的田间研究。转基因杨树在生长第1年、第2年和第3年的平均高度分别比对照高21.6%、36%和41%。随着时间的推移，转基因表达会影响植株的性状，导致叶片中蛋白质、总GS和铁氧还蛋白依赖性谷氨酸合成酶（Fd-GOGAT）增加。然而，在Rubisco （LSU）的大亚基丰度和水分含量方面，系间均未检测到差异。此外，树干中总多糖和木质素含量无显著差异。茎粗和树皮中蛋白质含量的分析表明，转基因的茎中积累了更高水平的氮储备。我们的研究结果表明，在短轮作人工林中，修改GS1的表达可能是一种有效的策略，以补充传统的树木育种。
In the present work the performance of transgenic poplars expressing a pine glutamine synthetase (GS) transgene was studied in natural conditions.A field study of eight independent transgenic lines and control plants was carried out for 3 yr in the province of Granada (Spain).Transgenic poplars reached average heights that were 21, 36 and 41% greater than control plants after the first, second and third year of growth, respectively. Transgene expression affected plant features with time resulting in increased protein, total GS and ferredoxin-dependent glutamate synthase (Fd-GOGAT) in leaves. However, neither differences in the large subunit of Rubisco (LSU) abundance nor water content were detected between lines. Furthermore, no significant differences were found in total polysaccharide and lignin content in tree trunks.The analyses of stem diameter, and protein contents in the bark suggest that higher levels of nitrogen reserves accumulated in the stem of transgenics. Our results suggest that modification of GS1 expression may be a useful strategy to complement traditional tree breeding in short rotation plantations.