Fibronectin, not laminin, mediates heparin-dependent heparin-binding growth factor type I binding to substrata and stimulation of endothelial cell growth.
Fibronectin, not laminin, mediates heparin-dependent heparin-binding growth factor type I binding to substrata and stimulation of endothelial cell growth.
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纤连蛋白（而不是层粘连蛋白）介导肝素依赖性肝素结合生长因子 I 型与基质的结合并刺激内皮细胞生长。
DOI：
10.1007/bf02623692
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Shi,EG
中科院分区：
文献类型：
--
作者：
Kan,M;Shi,EG
纤维连接蛋白和肝素结合生长因子（HBGF）是培养的内皮细胞生长所必需的。通过HBGF 1型（HBGF-1）刺激内皮细胞生长特别需要肝素或类似的糖胺聚糖。HBGF-1刺激的内皮细胞生长对纤连蛋白和肝素的需求可能是相关的。HBGF-1被吸收到内皮细胞的天然亚细胞基质中支持细胞生长。[125 I]HBGF-1特异性地与胶原-纤连蛋白-肝素的顺序重建基质结合，并且被吸收到重建基质的HBGF-1支持内皮细胞的生长。胶原-层粘连蛋白-肝素的重构基质既不支持[125 I]HBGF-1的结合，也不支持HBGF-1刺激的内皮细胞生长。[125 I]HBGF-1与内皮细胞单层上的肝素样位点和膜受体位点的结合动力学表明，亚细胞基质中的纤连蛋白-肝素样结合位点可能是活性HBGF-1与特异性细胞膜受体结合的强制性储存库。
Fibronectin and heparin-binding growth factors (HBGF) are essential for growth of cultured endothelial cells. The stimulation of endothelial cell growth by HBGF type one (HBGF-1) in particular requires heparin or a similar glycosaminoglycan. The requirement for fibronectin and heparin for HBGF-1-stimulated endothelial cell growth may be related. HBGF-1 absorbed to the natural subcellular matrix of endothelial cells supports cell growth. [125I]HBGF-1 specifically associates with a sequentially reconstituted matrix of collagen-fibronectin-heparin, and HBGF-1 absorbed to the reconstituted matrix supports growth of the endothelial cells. A reconstituted matrix of collagen-laminin-heparin neither supported binding of [125I]HBGF-1 nor HBGF-1-stimulated endothelial cell growth. Association kinetics of [125I]HBGF-1 to heparinlike sites and membrane receptor sites on endothelial cell monolayers suggest that fibronectin-heparinlike binding sites in the subcellular matrix may be an obligatory reservoir of active HBGF-1 that binds to specific cell membrane receptors.