Expulsion of allergen-containing materials from hydrated rye grass (Lolium perenne) pollen revealed by using immunogold field emission scanning and transmission electron microscopy
Expulsion of allergen-containing materials from hydrated rye grass (Lolium perenne) pollen revealed by using immunogold field emission scanning and transmission electron microscopy
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DOI：
10.1067/mai.2000.107044
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发表时间：
2000-06-01
影响因子：
14.2
通讯作者：
Reichelt, R
中科院分区：
文献类型：
--
作者：
Grote, M;Vrtala, S;Reichelt, R
背景：几项研究证实了大雨后草花粉引起的过敏性哮喘发作。据推测，这些哮喘发作可能是由于从花粉细胞质中释放出可吸入的含过敏原的颗粒。目的：在超微结构水平上研究新鲜收获和随后水化的黑麦草花粉中最有效和最常被识别的草花粉过敏原1组和5组的释放机制。用兔抗纯化重组1组和5组变应原血清，采用场发射扫描和透射免疫金电镜观察变应原从黑麦草花粉颗粒中释放到等渗水溶液或水中的情况。结果：花粉粒暴露在等渗水溶液中保持完整，并通过扩散释放过敏原。然而，在蒸馏物或雨水中水化的花粉粒会排出可呼吸大小的淀粉粒和细胞质碎片。在这些材料表面和内部观察到1组和5组过敏原。结论：将黑麦草花粉暴露于水中会导致含有亚细胞过敏原的可呼吸大小花粉成分的排出。因此，我们的超微结构数据支持这样的观点，即含有过敏原的可吸入花粉物质的释放可能是暴雨后哮喘发作的原因。
Background: Several studies demonstrated episodes of grass pollen-induced allergic asthma after heavy rainfalls. It has been hypothesized that these asthma attacks might be due to the release of respirable allergen-bearing particles from pollen cytoplasm.Objective: In this study we investigated the release mechanism of the most potent and frequently recognized grass pollen allergens, group 1 and group 5, from freshly harvested and subsequently hydrated rye grass pollen at the ultrastructural level,Methods: Rabbit antisera against purified recombinant group 1 and group 5 allergens were used to investigate, by using field emission scanning and transmission immunogold electron microscopy, the allergen release from rye grass pollen grains into isotonic aqueous solutions or water.Results: Pollen grains exposed to isotonic aqueous solutions remained intact and released allergens by means of diffusion. However, pollen grains hydrated in distilled mater or rainwater expelled starch grains and cytoplasmic debris of respirable size. Group 1 and group 5 allergens were observed on and within these materials.Conclusions: Exposure of rye grass pollen to water leads to an expulsion of subcellular allergen-containing pollen components of respirable size. Our ultrastructural data thus support the idea that this release of allergen-containing respirable pollen materials may be a cause of asthma attacks after heavy rainfalls.