Immunogenetic aspects of human thyroglobulin-reactive T cell lines and hybridomas.
Immunogenetic aspects of human thyroglobulin-reactive T cell lines and hybridomas.
复制标题
人甲状腺球蛋白反应性 T 细胞系和杂交瘤的免疫遗传学方面。
DOI：
10.1111/j.1744-313x.1990.tb00887.x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Kong,YM
中科院分区：
文献类型：
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作者：
Krco,CJ;Gores,A;David,CS;Kong,YM
在B10背景下比较了11种独立单倍型的人甲状腺球蛋白（Tg）致敏T细胞的体外增殖反应。B10.K和B10.S小鼠的反应性最强，而除B10.PL（H-2u）外，所有其他B10同类小鼠均为中等反应性。对人Tg体外激发的两个最佳应答者，即k和s单倍型，与对小鼠Tg诱导的实验性自身免疫性甲状腺炎（EAT）表现出H-2连锁易感性的应答者相同。由于在CBA（H-2k）小鼠中的过继转移研究表明，人类和小鼠Tgs上的共享表位具有致甲状腺作用，因此研究结果表明，可以在适当的（即EAT易感）小鼠品系中研究共享表位。因此，我们继续开发在B10.K和B10.S小鼠中产生人Tg的T细胞系和杂交瘤的方法，测试它们对异源Tgs的交叉反应性及其Ia限制性模式。通过使用来自重组菌株的抗原呈递细胞，我们鉴定了由I-A亚区单独编码的限制性元件和来自I-AI/I-E亚区的组合分子。
Thein vitroproliferative response of T cells primed with human thyroglobulin (Tg) was compared in 11 independent haplotypes on B10 background. B10.K and B10.S mice were the most responsive, whereas, with the exception of B10.PL (H‐2u, all other B10 congenics were intermediate responders. The two best responders toin vitrochallenge with human Tg, of thekand s haplotype, are the same as those showingH‐2‐linkedsusceptibility to induction of experimental autoimmune thyroiditis (EAT) with mouse Tg. Since shared epitopes on human and mouse Tgs have been shown to be thyroiditogenic by adoptive transfer studies in CBA (H‐2k) mice, the findings indicate that shared epitopes may be studied in appropriate (i.e. EAT‐susceptible) strains of mice. Therefore, we proceeded to develop methods to produce T‐cell lines and hybridomas to human Tg in B10.K and B10.S mice, test their cross‐reactivity to heterologous Tgs and their Ia restriction patterns. By using antigen‐presenting cells from recombinant strains, we identified restriction elements encoded by theI‐Asubregion alone and a combinatorial molecule from theI‐AI/I‐Esubregions.