Immunohistochemical study of 3 beta-hydroxysteroid dehydrogenase in sex cord-stromal tumors of the ovary.
Immunohistochemical study of 3 beta-hydroxysteroid dehydrogenase in sex cord-stromal tumors of the ovary.
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卵巢性索间质肿瘤中 3β-羟基类固醇脱氢酶的免疫组织化学研究。
DOI：
10.1097/00004347-199010000-00007
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Nagura,H
中科院分区：
文献类型：
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作者：
Sasano,H;Mason,JI;Sasaki,E;Yajima,A;Kimura,N;Namiki,T;Sasano,N;Nagura,H
将孕烯醇酮转化为孕酮的3[β]-羟基类固醇脱氢酶(3[β]-HSD)在18例卵泡瘤、23例纤维瘤、5例颗粒细胞瘤、5例硬化性间质瘤和2例类固醇细胞瘤中均有表达。卵巢癌和纤维瘤的连续切片中也进行了雌激素、孕激素和睾酮的免疫组织化学研究。在卵巢癌中，3[β]-HSD免疫反应仅见于黄素化的膜细胞和胞浆丰富的淡空泡型肿瘤细胞，而在梭形瘤细胞和胞浆中有少量至中度淡色空泡型的肿瘤性细胞中未见表达。除1例睾丸激素免疫反应外，未见类固醇免疫反应。纤维瘤中不存在类固醇或该酶的免疫反应。所有颗粒细胞瘤均未见肿瘤细胞表达3[β]-HSD。3[β]-HSD免疫反应阳性表达于类固醇细胞瘤细胞和2例硬化性间质瘤胞浆内有明显空泡的多角形肿瘤细胞。因此，3[β]-HSD可作为功能性卵巢肿瘤激素生成的免疫组织化学标志物。
3 [beta]-Hydroxysteroid dehydrogenase (3 [beta]-HSD), which converts pregnenolone to progesterone, was localized immunohistochemically in 18 thecomas, 23 fibromas, 5 granulosa-cell tumors, 5 sclerosing stromal tumors, and 2 steroid-cell tumors. Immunohistochemical study of estrogen, progesterone, and testosterone was also performed in serial sections of thecomas and fibromas. In thecomas, immunoreactivity of 3 [beta]-HSD was observed only in luteinized theca cells and thecomatous tumor cells with abundant pale to vacuolated cytoplasm but not in spindled tumor cells and thecomatous tumor cells with small to moderate amounts of pale to vacuolated cytoplasm. Immunoreactivity of steroids was not observed in thecomas except for testosterone immunoreactivity in one case. No immunoreactivity of steroids or the enzyme was present in fibromas. No tumor cells were positive for 3 [beta]-HSD in any of the cases of granulosa-cell tumor examined. Immunoreactivity of 3 [beta]-HSD was present in cells in steroid-cell tumors and polygonal tumor cells with prominent cytoplasmic vacuoles in two cases of sclerosing stromal tumor. Thus, 3 [beta]-HSD can be a good immunohistochemical marker of steroidogenesis in functioning ovarian neoplasms.