PROLIFERATION PATTERN OF POSTEMBRYONIC NEUROBLASTS IN THE BRAIN OF DROSOPHILA-MELANOGASTER
PROLIFERATION PATTERN OF POSTEMBRYONIC NEUROBLASTS IN THE BRAIN OF DROSOPHILA-MELANOGASTER
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DOI：
10.1016/0012-1606(92)90270-q
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发表时间：
1992-01-01
影响因子：
2.7
通讯作者：
HOTTA, Y
中科院分区：
文献类型：
--
作者：
ITO, K;HOTTA, Y
用5-溴-2 ' -脱氧尿苷（BrdU）标记DNA复制细胞，研究了黑腹果蝇（drosophila melanogaster）食道上神经节脑中央区胚胎后神经母细胞的时空增殖模式。刚孵化的幼虫每个大脑半球有五个增殖的神经母细胞：一个在大脑的腹外侧，另外四个在大脑的后背区域。分化的神经母细胞在一至二龄幼虫后期增加，达到每个半球约85个神经母细胞的平稳期。大多数神经母细胞在蛹形成（APF）后20-30小时停止分裂，而只有4个后背区神经母细胞在APF形成后85-90小时继续产生后代。在幼虫早期和蛹后期增殖的四种不同的神经母细胞是相同的；它们位于蘑菇体花萼上方的皮层（足柄体），其增殖周期是其他神经母细胞的两倍。它们的子神经元投射到蘑菇体神经堆中，因此很可能是凯尼恩细胞。四种神经母细胞（称为蘑菇体神经母细胞：MBNbs）的细胞周期在幼虫中期-蛹期早期相当稳定（1.1-1.5小时），而在此之前和之后都更长。整个胚胎和胚胎后发育过程中产生的MBNb后代总数估计为每个半球800-1200个。
The spatio-temporal proliferation pattern of postembryonic neuroblasts in the central brain region of the supraesophageal ganglion ofDrosophila melanogasterwas studied by labeling DNA replicating cells with 5-bromo-2′-deoxyuridine (BrdU). There are five proliferating neuroblasts per hemisphere in larvae just after hatching: one in the ventro-lateral, and the other four in the postero-dorsal region of the brain. Dividing neuroblasts increase during the late first-late second instar larval stages, reaching a plateau of about 85 neuroblasts per hemisphere. Most neuroblasts cease dividing 20–30 hr after puparium formation (APF), while only four in the postero-dorsal region continue making progenies until 85–90 hr APF. The four distinct neuroblasts proliferating in the early larval and late pupal stages are identical; they lie in the cortex above the calyces of the mushroom bodies (corpora pedunculata), proliferating over a period twice as long as that for the other neuroblasts. Their daughter neurons project into the mushroom body neuropile, and hence are likely to be the Kenyon cells. The cell-cycle period of the four neuroblasts (named mushroom body neuroblasts: MBNbs) is rather constant (1.1–1.5 hr) during the mid larval-early pupal stages and is longer before and after that. The total number of the MBNb progenies made throughout the embryonic and postembryonic development was estimated to be 800–1200 per hemisphere.