Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei
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DOI：
10.1038/28615
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发表时间：
1998-07-23
期刊：
影响因子：
64.8
通讯作者：
Yanagimachi, R
中科院分区：
文献类型：
--
作者：
Wakayama, T;Perry, ACF;Yanagimachi, R
直到最近，受精是产生可存活的哺乳动物后代的唯一方法，这一过程隐含地涉及雄性和雌性配子，然而，涉及胚胎或胎儿体细胞与去核卵母细胞融合的技术在产生克隆幼仔方面已经变得越来越成功（1-3）。多利羊（4）是通过绵羊乳腺来源的细胞与去核绵羊卵母细胞的电融合产生的。在这里，我们调查的因素，胚胎发育引入核体细胞（支持细胞，神经细胞和卵丘细胞）从成年小鼠到去核小鼠卵母细胞。我们发现，一些去核的卵母细胞接受塞尔托利或神经元核在体外开发和移植后，但没有开发超过8.5天后cobaly，然而，去核的卵母细胞接受卵丘核在体外开发的百分比很高。一旦转移，这些胚胎中的许多植入，虽然大多数随后被吸收，但有很大一部分（2 - 2.8%）发育到足月。这些实验表明，对于哺乳动物，来自终末分化的已知表型的成体体细胞的核被引入去核卵母细胞能够支持完全发育。
Until recently, fertilization was the only way to produce viable mammalian offspring, a process implicitly involving male and female gametes, However, techniques involving fusion of embryonic or fetal somatic cells with enucleated oocytes have become steadily more successful in generating cloned young(1-3). Dolly the sheep(4) was produced by electrofusion of sheep mammary-derived cells with enucleated sheep oocytes. Here we investigate the factors governing embryonic development by introducing nuclei from somatic cells (Sertoli, neuronal and cumulus cells) taken from adult mice into enucleated mouse oocytes. We found that some enucleated oocytes receiving Sertoli or neuronal nuclei developed in vitro and implanted following transfer, but none developed beyond 8.5 days post coitum; however, a high percentage of enucleated oocytes receiving cumulus nuclei developed in vitro. Once transferred, many of these embryos implanted and, although most were subsequently resorbed, a significant proportion (2 to 2.8%) developed to term. These experiments show that for mammals, nuclei from terminally differentiated, adult somatic cells of known phenotype introduced into enucleated oocytes are capable of supporting full development.