A Golgi‐localized glycosyltransferase, <scp>OsGT14;1</scp> , is required for growth of both roots and shoots in rice
A Golgi‐localized glycosyltransferase, <scp>OsGT14;1</scp> , is required for growth of both roots and shoots in rice
复制标题
水稻根和芽的生长都需要高尔基体定位的糖基转移酶 <scp>OsGT14;1</scp>
DOI：
10.1111/tpj.15897
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Ma Jian Feng
中科院分区：
文献类型：
--
作者：
Wang Peitong;Yamaji Naoki;Ma Jian Feng
糖基转移酶（GT）在植物中形成一个大家族，是合成各种多糖的重要酶，但只有少数成员得到了功能表征。本研究通过基因图谱突变筛选，发现具有短根表型的水稻突变体是由属于糖基转移酶基因家族 14的基因（OsGT14;1）发生移码突变引起的。进一步分析表明，与野生型水稻相比，该突变体茎秆脆，产量较低，但其根在矿物质吸收方面表现出相似的根结构和功能。 OsGT14;1在整个生育期各器官中广泛表达，其中在根、茎、节和皮中表达量较高。此外，OsGT14;1在这些器官的所有组织中都有表达。亚细胞观察表明OsGT14;1编码一种高尔基体定位蛋白。 OsGT14;1突变导致纤维素含量降低和半纤维素增加，但不改变根和芽细胞壁中的果胶。 OsGT14;1的敲除并不影响对有毒矿物元素（包括Al、As、Cd和盐胁迫）的耐受性，但确实增加了对低pH值的敏感性。总而言之，位于高尔基体的 OsGT14;1 通过影响纤维素合成是水稻根和芽生长所必需的。
Glycosyltransferases (GTs) form a large family in plants and are important enzymes for the synthesis of various polysaccharides, but only a few members have been functionally characterized. Here, through mutant screening with gene mapping, we found that anOryza sativa(rice) mutant with a short‐root phenotype was caused by a frame‐shift mutation of a gene (OsGT14;1) belonging to the glycosyltransferase gene family 14. Further analysis indicated that the mutant also had a brittle culm and produced lower grain yield compared with wild‐type rice, but the roots showed similar root structure and function in terms of the uptake of mineral nutrients.OsGT14;1was broadly expressed in all organs throughout the entire growth period, with a relatively high expression in the roots, stems, node I and husk. Furthermore,OsGT14;1was expressed in all tissues of these organs. Subcellular observation revealed thatOsGT14;1encoded a Golgi‐localized protein. Mutation ofOsGT14;1resulted in decreased cellulose content and increased hemicellulose, but did not alter pectin in the cell wall of roots and shoots. The knockout ofOsGT14;1did not affect the tolerance to toxic mineral elements, including Al, As, Cd and salt stress, but did increase the sensitivity to low pH. Taken together, OsGT14;1 located at the Golgi is required for growth of both roots and shoots in rice through affecting cellulose synthesis.