Gene promoter hypermethylation in oral rinses of leukoplakia patients -: a diagnostic and/or prognostic tool?
Gene promoter hypermethylation in oral rinses of leukoplakia patients -: a diagnostic and/or prognostic tool?
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DOI：
10.1016/s0959-8049(03)00550-1
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发表时间：
2003-11-01
影响因子：
8.4
通讯作者：
de Pancorbo, MM
中科院分区：
文献类型：
--
作者：
López, M;Aguirre, JM;de Pancorbo, MM
白斑病是最常见的口腔癌前病变，并显示了一个可变的恶性转化率。我们假设，检测分子改变，如启动子DNA的超甲基化，在口腔细胞学样本的患者在发展的原发性或复发性肿瘤的风险可能是一个有价值的诊断和预后工具，在这些病变的管理。分析了两组具有不同发展口腔鳞状细胞癌（OSCC）风险的患者。从每位患者的口腔冲洗液中提取DNA。使用甲基化特异性聚合酶链反应（MSP）测定p16、p14和MGMT基因启动子的甲基化状态。在44%和56%的口腔样本中分别观察到p16和MGMT的甲基化。只有12%的病例显示p14甲基化。DNA高甲基化在有口腔鳞癌病史的患者中更常见。DNA启动子高甲基化在口腔癌发生的早期是常见的，在晚期更是如此。MSP是一种非侵入性、高灵敏度的技术，可用于监测口腔癌前病变和癌性病变。(C)2003 Elsevier Ltd.保留所有权利。
Leukoplakia is the most frequent oral precancerous lesion and shows a variable rate of malignant transformation. We hypothesised that the detection of molecular alterations, like the promoter hypermethylation of DNA, in oral cytological samples from patients at risk of developing primary or recurrent tumours could be a valuable diagnostic and prognostic tool in the management of these lesions. Two groups of patients with differing risks of developing oral squamous cell carcinoma (OSCC) were analysed. DNA was extracted from the oral rinse of each patient. The methylation status of the p16, p14 and MGMT gene promoters was determined using a methylation-specific polymerase chain reaction (MSP). Methylation of p16 and MGMT was observed in 44 and 56% of the oral samples, respectively. Only 12% of the cases showed p14 methylation. DNA hypermethylation was more frequent in patients with previous OSCC. DNA promoter hypermethylation is frequent during early oral carcinogenesis and even more so in the later stages. MSP using oral rinses is a non-invasive and highly sensitive technique which could be used to monitor patients with precancerous and cancerous oral lesions. (C) 2003 Elsevier Ltd. All rights reserved.