An in vivo microdialysis characterization of extracellular dopamine and GABA in dorsolateral striatum of awake freely moving and halothane anaesthetised rats.
An in vivo microdialysis characterization of extracellular dopamine and GABA in dorsolateral striatum of awake freely moving and halothane anaesthetised rats.
复制标题
清醒自由活动和氟烷麻醉大鼠背外侧纹状体细胞外多巴胺和 GABA 的体内微透析表征。
DOI：
10.1016/0165-0270(90)90047-j
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发表时间：
1990
影响因子：
3
通讯作者：
Ungerstedt,U
中科院分区：
文献类型：
--
作者：
Osborne,PG;O'Connor,WT;Drew,KL;Ungerstedt,U
本研究描述了在急性（2.5 h）和慢性（1天、2天和4天）植入探针后，利用体内微透析技术从大鼠背外侧纹状体中提取细胞外多巴胺（DA）和γ-氨基丁酸（GABA）的系统表征结果。钠通道阻滞剂河豚毒素（TTX 10-6M）和无Ca2+的Ringers灌注介质灌注表征了DA和GABA溢流的电压和钙依赖性。此外，我们还研究了氟烷麻醉对这些神经递质物质对TTX和无Ca2+灌注介质的反应性的影响。在所有组中，灌注TTX可使基础DA水平降低至少60%。在探针植入24 h后，氟烷麻醉大鼠ttx诱导的下降最为明显。各组间GABA对TTX输注的反应性不同。在急性植入氟烷麻醉的大鼠中，灌注TTX未改变基础GABA水平，而在其余组中至少观察到35%的降低。在植入探针2天后清醒的大鼠中，从灌注介质中去除和替换Ca2+导致基础DA可逆降低87%。此外，基础GABA水平降低了52%。在将无Ca2+灌注介质替换为正常灌注介质后1.5 h，这种下降被延迟且不逆转，尽管基础GABA水平在第二天恢复到实验前水平。本研究表明纹状体DA和GABA对电压依赖性（TTX）和囊泡释放（Ca2+无灌注）的反应性受到透析纤维植入后经过的时间长度和动物的意识状态的影响。
This study describes the results of a systematic characterization of extracellular dopamine (DA) and γ-aminobutyric acid (GABA) recovered from dorsolateral striatum using in vivo microdialysis in rats following acute (2.5 h) and chronic (1 day, 2 day and 4 day) implantation of the probe. The voltage and calcium dependence of DA and GABA overflow was characterised by perfusion with the sodium channel blocker tetrodotoxin (TTX 10-6M) and with Ca2+-free Ringers perfusion medium. In addition, the effect of halothane anaesthesia on the responsiveness of these neurotransmitter substances to TTX and Ca2+-free perfusion medium was investigated. Perfusion with TTX decreased basal DA levels by at least 60% in all groups. The TTX-induced decrease was most profound in halothane-anaesthetised rats, 24 h after implantation of the probe. Responsiveness of GABA to TTX infusion was different between the groups. In acutely implanted halothane-anaesthetised rats basal GABA levels were unaltered by perfusion with TTX while in the remaining groups at least a 35% reduction was observed. In awake rats 2 days following implantation of the probe removal and replacement of the Ca2+from the perfusion medium resulted in a reversible reduction of basal DA by 87%. In addition, basal GABA levels were decreased by 52%. This decrease was delayed and was not reversed 1.5 h after the Ca2+-free perfusion medium was replaced with normal perfusion medium although basal GABA levels returned to pre-experimental levels by the following day. The present study demonstrates that the responsiveness of striatal DA and GABA to manipulations of both voltage-dependent (TTX) and vesicular release (Ca2+-free perfusion) is influenced by the length of time elapsed after the implantation of the dialysis fiber and the conscious state of the animal.