DETECTION OF CLOSTRIDIUM-BOTULINUM TYPE-A TOXIN BY ENZYME-LINKED IMMUNOSORBENT-ASSAY WITH ANTIBODIES PRODUCED IN IMMUNOLOGICALLY TOLERANT ANIMALS
DETECTION OF CLOSTRIDIUM-BOTULINUM TYPE-A TOXIN BY ENZYME-LINKED IMMUNOSORBENT-ASSAY WITH ANTIBODIES PRODUCED IN IMMUNOLOGICALLY TOLERANT ANIMALS
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DOI：
10.1128/jcm.19.5.645-648.1984
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发表时间：
1984-01-01
影响因子：
9.4
通讯作者：
BARTLETT, JG
中科院分区：
文献类型：
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作者：
DEZFULIAN, M;BARTLETT, JG
免疫耐受是一种对外来物质（抗原）无反应的状态，它可以在人类和动物物种中发展为生命早期持续接触抗原的结果。该原理用于制备A型肉毒杆菌毒素抗体。通过选择性抑制兔对不想要的抗原的免疫反应，随后用类毒素免疫，产生一种特定的a型抗毒素，而不需要纯化毒素。尽管与B型肉毒杆菌有交叉反应，但A型抗毒素在其他方面是特异性的，因为它与B型肉毒杆菌的非毒素变异、任何其他肉毒杆菌（C、D、E、F和G）的培养滤液都不发生反应，也与18种其他梭菌（包括产孢梭菌）不发生反应。利用该抗毒素，建立了a型肉毒杆菌毒素的ELISA检测方法。
Immunological tolerance is a state of unresponsiveness to foreign substances (antigens) which can develop in human and animal species as the result of continued exposure to antigens early in life. This principle was used for the preparation of antibodies against C. botulinum type A toxin. By selective suppression of the immunological response of rabbits to unwanted antigens and subsequent immunization with a toxoid, a specific type A antitoxin was produced without the need to purify the toxin. Despite cross-reactivity with C. botulinum type B, the type A antitoxin was otherwise specific since it did not react with culture filtrates of nontoxigenic variants of type B, any other C. botulinum type (C, D, E, F and G), nor with 18 other Clostridium spp., including C. sporogenes. Using this antitoxin, a sensitive ELISA for detection of C. botulinum type A toxin was developed.