Engineering cooperativity in biomotor-protein assemblies
Engineering cooperativity in biomotor-protein assemblies
复制标题
DOI：
10.1126/science.1122125
复制
发表时间：
2006-03-10
期刊：
影响因子：
56.9
通讯作者：
Tirrell, DA
中科院分区：
文献类型：
--
作者：
Diehl, MR;Zhang, KC;Tirrell, DA
A biosynthetic approach was developed to control and probe cooperativity in multiunit biomotor assemblies by linking molecular motors to artificial protein scaffolds. This approach provides precise control over spatial and elastic coupling between motors. Cooperative interactions between monomeric kinesin-1 motors attached to protein scaffolds enhance hydrolysis activity and microtubule gliding velocity. However, these interactions are not influenced by changes in the elastic properties of the scaffold, distinguishing multimotor transport from that powered by unorganized monomeric motors. These results highlight the role of supramolecular architecture in determining mechanisms of collective transport.
