Sequence organization of feline leukemia virus DNA in infected cells.
Sequence organization of feline leukemia virus DNA in infected cells.
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受感染细胞中猫白血病病毒 DNA 的序列组织。
DOI：
10.1093/nar/8.15.3287
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发表时间：
1980
影响因子：
14.9
通讯作者：
Davidson,N
中科院分区：
文献类型：
--
作者：
Mullins,JI;Casey,JW;Nicolson,MO;Davidson,N
在实验性感染Gardner-Arnstein FeLV毒株后，已推导出人RD细胞中发现的未整合和整合FeLV病毒DNA的限制性酶切位点图谱。通过单酶和双酶切对限制性片段进行排序，然后进行Southern转移（1）并与32 P标记的病毒cDNA探针杂交。用3′端特异性杂交探针对病毒RNA的5′端和3′端进行定位。未整合病毒DNA的主要形式是一个8.7kb的线性DNA分子，带有一个450 bp的直接长末端冗余（LTR），来自5′和3′病毒RNA序列。还检测到长度为8.7 kb和8.2 kb的较小环状形式，较大的可能含有两个相邻的LTR拷贝，较小的可能含有一个LTR拷贝。FeLV的整合拷贝与未整合的线性形式共线，并包含在每个LTR中发现的Kpn I和SmaI位点。
A restriction site map has been deduced of unintegrated and integrated FeLV viral DNA found in human RD cells after experimental infection with the Gardner-Arnstein strain of FeLV. Restriction fragments were ordered by single and double enzyme digests followed by Southern transfer (1) and hybridization with32P-labeled viral cDNA probes. The restriction map was oriented with respect to the 5′ and 3′ ends of viral RNA by using a 3′ specific hybridization probe. The major form of unintegrated viral DNA found was a 8.7 kb linear DNA molecule bearing a 450 bp direct long terminal redundancy (LTR) derived from both 5′ and 3′ viral RNA sequences. Minor, circular forms, 8.7 kb and 8.2 kb in length were also detected, the larger one probably containing two adjacent copies of the LTR and the smaller one containing one copy of the LTR. Integrated copies of FeLV are colinear with the unintegrated linear form and contain the KpnI and SmaI sites found in each LTR.