Cortical Synaptic AMPA Receptor Plasticity during Motor Learning
Cortical Synaptic AMPA Receptor Plasticity during Motor Learning
复制标题
运动学习过程中皮质突触 AMPA 受体可塑性
DOI：
10.1016/j.neuron.2019.12.005
复制
发表时间：
2020-03-04
期刊：
影响因子：
16.2
通讯作者：
Huganir, Richard L.
中科院分区：
文献类型：
--
作者：
Roth, Richard H.;Cudmore, Robert H.;Huganir, Richard L.
通过AMPA受体（AMPAR）的运输来调节突触强度是突触可塑性、学习和记忆的基本机制。然而，体内AMPAR运输的动力学及其与学习的相关性尚未得到解决。在这里，我们在体内使用双光子显微镜可视化表面AMPAR在小鼠皮层在收购前肢达到任务。日常训练导致运动皮层中空间聚集的树突棘亚组的AMPAR水平增加。令人惊讶的是，我们还观察到视觉皮层中脊柱AMPAR水平的增加。在那里，突触增强取决于运动训练期间视觉输入的可用性，并且视觉皮层活动的光遗传学抑制损害任务表现。这些结果表明，运动学习诱导广泛的皮层突触增强通过增加AMPAR的净贩运到脊柱，包括在非运动脑区域。
Modulation of synaptic strength through trafficking of AMPA receptors (AMPARs) is a fundamental mechanism underlying synaptic plasticity, learning, and memory. However, the dynamics of AMPAR trafficking in vivo and its correlation with learning have not been resolved. Here, we used in vivo two-photon microscopy to visualize surface AMPARs in mouse cortex during the acquisition of a forelimb reaching task. Daily training leads to an increase in AMPAR levels at a subset of spatially clustered dendritic spines in the motor cortex. Surprisingly, we also observed increases in spine AMPAR levels in the visual cortex. There, synaptic potentiation depends on the availability of visual input during motor training, and optogenetic inhibition of visual cortex activity impairs task performance. These results indicate that motor learning induces widespread cortical synaptic potentiation by increasing the net trafficking of AMPARs into spines, including in non-motor brain regions.