Physiological, Metabolic, and Transcriptomic Analyses Reveal the Responses of Arabidopsis Seedlings to Carbon Nanohorns
Physiological, Metabolic, and Transcriptomic Analyses Reveal the Responses of Arabidopsis Seedlings to Carbon Nanohorns
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生理、代谢和转录组分析揭示拟南芥幼苗对碳纳米角的反应
DOI：
10.1021/acs.est.9b07133
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发表时间：
2020
影响因子：
11.4
通讯作者：
Xu Jin
中科院分区：
文献类型：
--
作者：
Sun Liangliang;Wang Ruting;Ju Qiong;Xu Jin
碳基纳米材料在纳米农业中具有潜在的应用前景。然而，单壁碳纳米角（SWCNH）介导的植物生长的生理和分子机制仍不清楚。在这里，我们研究了SWCNHs对生长在1/4强度Murashige和Skoog培养基中的拟南芥的影响，通过生理，遗传和分子分析。0.1 mg/L的SWCNHs处理促进了初生根和侧根的形成，50和100 mg/L的SWCNHs处理抑制了初生根的形成。0.1 mg/L SWCNHs处理增加了分生区和伸长区的长度，转录组学和遗传分析证实了SWCNHs对根尖干细胞生态位活性和分生细胞分裂潜力的积极影响。在0.1 mg/L SWCNH处理的幼苗中，YUC 3和YUC 5的表达增加以及PIN 2丰度的增加促进了PR生长和LR发育。代谢组学分析表明，SWCNHs改变了糖，氨基酸和有机酸的水平，这表明SWCNHs重编程的碳/氮代谢在植物中。SWCNH还通过增加几种次生代谢产物的水平来调节植物的生长和发育;转录组学分析进一步支持了这些结果。目前的结果对于SWCNH在农业纳米技术中的持续使用具有价值，这些分子方法可以作为研究纳米材料对植物影响的例子。
Carbon-based nanomaterials have potential applications in nanoenabled agriculture. However, the physiological and molecular mechanisms underlying single-walled carbon nanohorn (SWCNH)-mediated plant growth remain unclear. Here, we investigated the effects of SWCNHs onArabidopsisgrown in1/4-strength Murashige and Skoog medium via physiological, genetic, and molecular analyses. Treatment with 0.1 mg/L SWCNHs promoted primary root (PR) growth and lateral root (LR) formation; 50 and 100 mg/L SWCNHs inhibited PR growth. Treatment with 0.1 mg/L SWCNHs increased the lengths of the meristematic and elongation zones, and transcriptomic and genetic analyses confirmed the positive effects of SWCNHs on root tip stem cell niche activity and meristematic cell division potential. Increased expression ofYUC3andYUC5and increased PIN2 abundance improved PR growth and LR development in 0.1 mg/L SWCNH-treated seedlings. Metabolomic analyses revealed that SWCNHs altered the levels of sugars, amino acids, and organic acids, suggesting that SWCNHs reprogrammed carbon/nitrogen metabolism in plants. SWCNHs also regulate plant growth and development by increasing the levels of several secondary metabolites; transcriptomic analyses further supported these results. The present results are valuable for continued use of SWCNHs in agri-nanotechnology, and these molecular approaches could serve as examples for studies on the effects of nanomaterials in plants.