Loss of genetic variation at microsatellite loci in hatchery produced abalone in Australia (Haliotis rubra) and South Africa (Haliotis midae)
Loss of genetic variation at microsatellite loci in hatchery produced abalone in Australia (Haliotis rubra) and South Africa (Haliotis midae)
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DOI：
10.1016/j.aquaculture.2003.09.037
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发表时间：
2004-04-26
期刊：
影响因子：
4.5
通讯作者：
Elliott, NG
中科院分区：
文献类型：
--
作者：
Evans, B;Bartlett, J;Elliott, NG
利用微卫星DNA标记分别研究了南非和澳大利亚的大鲍和红鲍养殖群体的遗传多样性水平。所研究的养殖群体是野生捕捞亲鱼的F1代。所有种群的遗传多样性都有所下降，以每个位点的等位基因数（35-62%的等位基因丢失）衡量，与各自亲鱼收集区的野生种群相比。然而，没有相关的杂合性丢失。在这两个物种中观察到扇和野生样品之间的等位基因频率的变化。平均水平的遗传相关的培养H。midae与0无显著差异，而培养的H. rubra明显更高。有效种群大小的估计值。西海岸养殖场的midae亲鱼在75.3（SD +/- 57.6）和43.5（+/- 29.8）之间，东海岸养殖场在18.5（+/- 8.4）和16.8（+/- 8.0）之间。在两个农场样本中观察到的等位基因损失显著大于预期，这是由于基于这种有效种群大小估计的遗传漂变。本文还研究了一个农场样地的有效种群大小。Rubra估计在27.2（+/- 3.8）和22.4（+/- 4.7）之间。由于遗传漂变，在这种情况下观察到的等位基因损失并不显著大于预期。(C)2004 Elsevier B. V.保留所有权利。
Microsatellite DNA markers were used to investigate levels of genetic diversity within cultured populations of Haliotis midae and Haliotis rubra in South Africa and Australia, respectively. The cultured populations examined were F1 progeny of wild caught broodstock. All populations show a decline in genetic diversity, measured as the number of alleles per locus (35-62% allele loss) when compared to wild stocks in the area of respective broodstock collection. There was, however, no associated loss of heterozygosity. Changes in the frequency of alleles between fanned and wild samples were observed in both species. Mean levels of genetic relatedness for the cultured H. midae were not significantly different to zero, while those for the cultured H. rubra were significantly higher. The estimated effective population size of H. midae broodstock was between 75.3 (SD +/- 57.6) and 43.5 (+/- 29.8) for a west coast farm and between 18.5 (+/- 8.4) and 16.8 (+/- 8.0) for an east coast farm. The observed loss of alleles in both farm samples was significantly greater than that expected due to genetic drift based on such effective population size estimates. The effective population size of a farm sample of H. rubra was estimated at between 27.2 (+/- 3.8) and 22.4 (+/- 4.7). The observed loss of alleles in this instance was not significantly greater than expected due to genetic drift. (C) 2004 Elsevier B.V. All rights reserved.