Spatiotemporal micropatterning of cells on arbitrary substrates
Spatiotemporal micropatterning of cells on arbitrary substrates
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DOI：
10.1021/ac062371p
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发表时间：
2007-06-01
影响因子：
7.4
通讯作者：
Beebe, David J.
中科院分区：
文献类型：
--
作者：
Abhyankar, Vinay V.;Beebe, David J.
本文提出了一个基于几何约束、离散细胞悬浮液滴和控制细胞沉降的易于访问的模式平台，该平台提供了时间和空间的模式能力。作为一个示范，单细胞（和头）悬浮液以及多细胞人类胚胎干细胞菌落在时空上被图案化到任意基质上。这些底物包括组织培养表面、细胞单层、蛋白包被表面和3D凝胶基质。还演示了可溶性因子梯度的产生。这种方法是完全被动的，不需要外部电源。时空模式为未来探索细胞-细胞信号传导和细胞反应之间的时间依赖性关系的生物学研究提供了基础。
This paper presents a readily accessible patterning platformbased upon geometric constraints, discrete cell suspension droplets, and controlled cell settlingthat provides both temporal and spatial patterning capabilities. As a demonstration, single-cell (and bead) suspensions as well as multicellular human embryonic stem cell colonies are spatiotemporally patterned onto arbitrary substrates. These substrates include tissue culture surfaces, cell monolayers, protein-coated surfaces, and 3D gel matrices. The generation of soluble factor gradients is also demonstrated. This method is completely passive and does not require external power sources. Spatiotemporal patterning provides a foundation for future biological studies that explore the time-dependent relationships between cell-cell signaling and cellular responses.