cDNA cloning and expression of human activin βE subunit
cDNA cloning and expression of human activin βE subunit
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DOI：
10.1016/s0303-7207(02)00157-0
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发表时间：
2002-08-30
影响因子：
4.1
通讯作者：
Hasegawa, Y
中科院分区：
文献类型：
--
作者：
Hashimoto, O;Tsuchida, K;Hasegawa, Y
我们用聚合酶链反应（PCR）技术从肝脏cDNA文库中克隆了人激活素PE亚基cDNA。推导的氨基酸序列与小鼠和大鼠激活素β E亚基的同源性分别为97%和96%。在哺乳动物细胞中表达在COOH末端用Myc和多组氨酸残基标记的人激活素β E亚基，并作为二硫键连接的同源二聚体蛋白分泌到培养基中。我们还发现，人激活素β E蛋白可以结合卵泡抑素，激活素结合蛋白。北方杂交分析表明，该基因主要在人肝脏中表达，转录产物大小为2.7kb。这些发现表明激活素E（二聚体蛋白）可能在人类中发挥作用。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
We cloned human activin PE subunit cDNA from a liver cDNA library using the polymerase chain reaction (PCR) technique. The deduced amino acid sequence was 97 and 96% homologous to the mouse and rat activin betaE subunits. Human activin betaE subunit tagged with Myc and polyhistidine residues at the COOH terminus was expressed in mammalian cells and secreted into the medium as a disulphide-linked homodimer protein. We also found that the human activin betaE protein could bind to follistatin, an activin-binding protein. Northern blot analysis showed that this gene was expressed as a major transcript of 2.7 kb predominantly in human liver. These findings suggest that activin E (dimeric protein) may play a role in humans. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.