Electrospun collagen-chitosan nanofiber: A biomimetic extracellular matrix for endothelial cell and smooth muscle cell
Electrospun collagen-chitosan nanofiber: A biomimetic extracellular matrix for endothelial cell and smooth muscle cell
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DOI：
10.1016/j.actbio.2009.07.024
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发表时间：
2010-02-01
影响因子：
9.7
通讯作者：
Cui, F. Z.
中科院分区：
文献类型：
--
作者：
Chen, Z. G.;Wang, P. W.;Cui, F. Z.
采用静电纺丝技术将胶原蛋白和壳聚糖在1，1，1，3，3，3-六氟异丙醇/三氟乙酸（v/v，90/10）混合溶液中共混，制备了具有生物相容性的仿生组织工程纳米结构支架。用扫描电镜（SEM）观察了胶原蛋白-壳聚糖纳米纤维的形貌，并用戊二醛（GTA）蒸汽交联法对其进行了稳定。傅立叶变换红外光谱分析表明，胶原-壳聚糖纳米纤维没有显着变化，但增强了稳定性后，由GTA蒸汽交联。X射线衍射分析表明，胶原蛋白和壳聚糖分子链在静电纺丝和交联过程中不能结晶，纤维呈无定形结构。采用差示扫描量热法和拉伸试验研究了胶原蛋白-壳聚糖电纺纤维的热行为和力学性能。为了检测电纺纤维的生物相容性，还通过SEM和甲基噻唑四氮唑测试研究了细胞在纳米纤维支架上的行为。结果表明，内皮细胞和平滑肌细胞在血管上或血管内增殖良好。结果表明，胶原-壳聚糖复合基质可能是一个更好的候选人组织工程在生物医学应用，如支架。(C)2009 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
Electrospinning of collagen and chitosan blend solutions in a 1,1,1,3,3,3-hexafluoroisopropanol/trifluoroacetic acid (v/v, 90/10) mixture was investigated for the fabrication of a biocompatible and biomimetic nanostructure scaffold in tissue engineering. The morphology of the electrospun collagen-chitosan nanofibers was observed by scanning electron microscopy (SEM) and stabilized by glutaraldehyde (GTA) vapor via crosslinking. Fourier transform infrared spectra analysis showed that the collagen-chitosan nanofibers do not change significantly, except for enhanced stability after crosslinking by GTA vapor. X-ray diffraction analysis implied that both collagen and chitosan molecular chains could not be crystallized in the course of electrospinning and crosslinking, and gave an amorphous structure in the nanofibers. The thermal behavior and mechanical properties of electrospun collagen-chitosan fibers were also studied by differential scanning calorimetry and tensile testing, respectively. To assay the biocompatibility of electrospun fibers, cellular behavior on the nanofibrous scaffolds was also investigated by SEM and methylthiazol tetrazolium testing. The results show that both endothelial cells and smooth muscle cells proliferate well on or within the nanofiber. The results indicate that a collagen-chitosan nanofiber matrix may be a better candidate for tissue engineering in biomedical applications such as scaffolds. (C) 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.