Monolayer rubrene functionalized graphene-based eletrochemiluminescence biosensor for serum cystatin C detection with immunorecognition-induced 3D DNA machine
Monolayer rubrene functionalized graphene-based eletrochemiluminescence biosensor for serum cystatin C detection with immunorecognition-induced 3D DNA machine
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单层红荧烯功能化石墨烯电化学发光生物传感器，用于免疫识别诱导的 3D DNA 机器检测血清胱抑素 C
DOI：
10.1016/j.bios.2018.12.009
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发表时间：
2019
影响因子：
12.6
通讯作者：
Ding Shijia
中科院分区：
文献类型：
--
作者：
Zhao Min;Bai Lijuan;Cheng Wei;Duan Xiaolei;Wu Haiping;Ding Shijia
在典型的 ECL 反应中，只有电化学发光 (ECL) 纳米材料的表面分子才是最具反应活性的物质。在此，将单层红荧烯组装在石墨烯片的表面上，通过最大化表面红荧烯分子，获得具有强ECL发射的单层红荧烯功能化石墨烯复合物（G/mRub）。基于 G/mRub 作为强 ECL 发射器，开发了一种超灵敏的“开关”生物传感器，借助新型免疫识别诱导的无酶 3D DNA 机器来检测人血清中的半胱氨酸蛋白酶抑制剂 C (Cys C)。得益于G/mRub强烈的ECL发射和3D DNA机器的高效信号放大，所建立的生物传感器实现了Cys C检测的高灵敏度，线性范围为1.0fgmL−1至10ngmL−1，检测限低至0.38fgmL−1。此外，采用这种无酶生物传感方法成功检测了人血清中Cys C的浓度。因此，基于G/mRub的ECL生物传感器可能为临床诊断中的蛋白质检测提供潜在的工具，并为制备高性能发光纳米材料提供新途径。
Only surficial molecules of eletrochemiluminescent (ECL) nanomaterials are the most reactive species in the typical ECL reaction. Herein, monolayer rubrene was assembled on the surface of graphene sheet to obtain monolayer rubrene functionalized graphene composite (G/mRub) with strong ECL emission by maximizing the surficial rubrene molecules. Based on G/mRub as the strong ECL emitter, an ultrasensitive “on-off” biosensor was developed to detect cystatin C (Cys C) in human serum with the help of a novel immunorecognition-induced enzyme-free 3D DNA machine. Benefiting from the strong ECL emission of G/mRub and the efficient signal amplification of 3D DNA machine, the established biosensor achieved high sensitivity for Cys C detection with linear range from 1.0 fg mL−1to 10 ng mL−1and limit of detection down to 0.38 fg mL−1. In addition, this enzyme-free biosensing method was adopted to successfully detect the concentration of Cys C in human serum. Therefore, the G/mRub based ECL biosensor might provide a potential tool for protein detection in clinical diagnosis and a new avenue to prepare high-performance luminescent nanomaterials.