Quantitative lymph imaging for assessment of lymph function using indocyanine green fluorescence lymphography
Quantitative lymph imaging for assessment of lymph function using indocyanine green fluorescence lymphography
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DOI：
10.1016/j.ejvs.2008.04.013
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发表时间：
2008-08-01
影响因子：
5.7
通讯作者：
Konno, H.
中科院分区：
文献类型：
--
作者：
Unno, N.;Nishiyama, M.;Konno, H.
目的：一种新的诊断成像技术，可以评估淋巴功能是需要作为一个筛选测试在日常实践中。本研究评估了吲哚菁绿色（ICG）荧光淋巴造影术在无腿部水肿的受试者中的应用。随后，通过用红外光相机观察其荧光信号来追踪ICG染料从注射部位到腹股沟的移动。测量染料到达膝盖和腹股沟的时间（到达膝盖的通过时间：TT（K），到达腹股沟的通过时间：TT（G））。10名健康志愿者分别在站立、仰卧、按摩站立和坐位进行功率为50 W、转速为50 rpm的自行车运动，每隔14 d测量TTG。结果：站立时左右腿TTG平均值分别为357 289和653 564 s。与站立位TTG相比，所有其他条件均缩短TTG。在另外17例无下肢水肿的受试者中，我们比较了ICG荧光淋巴造影和动态淋巴造影获得的通过时间。ICG荧光淋巴造影与动态淋巴造影的通过时间有显著相关性（r（2）= 0.64，p < 0.01）。结论：ICG荧光淋巴造影有可能成为评价淋巴功能的一种新的淋巴显像技术。(C)2008年欧洲血管外科学会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: A new diagnostic imaging technique that can assess lymph function is needed as a screening test in daily practice. This study assessed the use of indocyanine green (ICG) fluorescence lymphography in subjects without leg oedema.Methods: 0.3 ml of ICG (0.5 %) was injected subcutaneously at the dorsum of the foot. Subsequently, the movement of ICG dye from the injection site to the groin was traced by visualizing its fluorescence signal with an infrared light camera. The time for the dye to reach the knee and groin were measured (Transit time to knee: TT(K), Transit time to groin: TT(G)). TTG was measured while standing, lying at a supine position, standing with massage, and sitting while using a cycle ergometer exercise at an intensity of 50 W at 50 rpm in ten healthy volunteers at intervals of 14 days.Results: Mean TTG during standing was 357 289 and 653 564 seconds for the right and left legs respectively. Compared to TTG in the standing position, all other conditions shortened TTG. In another seventeen subjects without leg oedema, we compared transit time obtained with ICG fluorescence lymphography to that with dynamic lymphoscintigraphy. A significant correlation between transit time measured with ICG lymphography and dynamic lymphoscintigraphy was identified (r(2) = 0.64, p < 0.01).Conclusions: ICG fluorescence lymphography has the potential to become an alternative lymphatic imaging technique to assess lymph function. (C) 2008 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.