Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China
Molecular identification and characterization of Anaplasma capra and Anaplasma platys-like in Rhipicephalus microplus in Ankang, Northwest China
复制标题
西北安康地区微小扇头蜱无形体和类无形体的分子鉴定与表征
DOI：
10.1186/s12879-019-4075-3
复制
发表时间：
2019-05-17
影响因子：
3.7
通讯作者：
Zhou, En-Min
中科院分区：
文献类型：
--
作者：
Guo, Wen-Ping;Zhang, Bing;Zhou, En-Min
无形体属（Anaplasma）的4种无形体是近年来出现的人畜共患病原体，主要由蜱类传播，引起兽医和公众的关注。在这里，我们进行了分子调查，在安康，中国西北地区。方法硬蜱收集和鉴定，采用形态学和分子生物学方法。使用巢式聚合酶链反应检测人类致病性无形体属。几乎完整的rrs，gltA，和groEL基因序列揭示无浆虫物种进行了扩增和测序，以确定其分子特征和它们的genogeny.ResultsAll蜱收集在安康属于微小扇头蜱。与A. platys和A.在这些蜱中检测到山羊。本研究中发现的新的未分类无形体菌株在A.扁形虫及相关株群。A的启示。在这项研究中发现的山羊菌株与在人类和其他脊椎动物中检测到的菌株最密切相关。capra是R.微小蜱在以前未被认识的流行地区。我们还发现了一种新的未分类的无形体属物种，与A.扁平的由这两种无形体引起的人类无形体病的流行病学应在未来的研究中进行评估。
BackgroundFour species within Anaplasma genus are emerging zoonotic pathogens, which are transmitted by ticks and generate veterinary and public health concerns. Here, we performed a molecular survey of Anaplasma in Ankang, Northwest China.MethodsHard ticks were collected and identified using morphological and molecular methods. Human-pathogenic Anaplasma species were tested using nested polymerase chain reaction. The nearly complete rrs, gltA, and groEL genes sequences from revealed Anaplasma species were amplified and sequenced to determine their molecular characteristics and their phylogeny.ResultsAll ticks collected in Ankang belonged to the Rhipicephalus microplus. Novel unclassified Anaplasma strains genetically related to A. platys and A. capra were detected in these ticks. Co-infection of these two organisms was also found. The novel unclassified Anaplasma strains identified in this study formed a distinct phylogenetic lineage based on the groEL gene and two lineages based on the gltA gene within A. platys and related strains group. The revealed A. capra strains identified in this study were most closely related to those detected in humans and other vertebrate animals.ConclusionWe revealed the presence of A. capra, a novel human pathogens in R. microplus ticks in previously unrecognized endemic regions. We also detected a novel unclassified Anaplasma species genetically related to A. platys. The epidemiology of anaplasmosis caused by these two Anaplasma species in humans should be assessed in future studies.