wrinkled1:: A novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism
wrinkled1:: A novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism
复制标题
DOI：
10.1104/pp.118.1.91
复制
发表时间：
1998-09-01
期刊：
影响因子：
7.4
通讯作者：
Benning, C
中科院分区：
文献类型：
--
作者：
Focks, N;Benning, C
在拟南芥种子发育中的油沉积过程中，光合作用产物以碳水化合物的形式输入到胚胎中，并转化为三酰甘油。为了鉴定这一过程所必需的基因，并研究油脂积累的发育调控的分子基础，分离了产生皱纹、不完全充满的种子的突变体。发现了一个新的突变基因座，皱纹1(Wri1)，它位于3号染色体的底部，导致种子含油量下降80%。野生型和纯合型wri1突变植株或成熟植株难以区分。然而，发育中的纯合wri1种子在三酰甘油中加入蔗糖和葡萄糖而受到损害，但加入丙酮酸和乙酸酯的速度加快。由于WRI1纯合种子发育过程中几种糖酵解酶，特别是己糖激酶和焦磷酸依赖的磷酸果糖激酶的活性降低，因此WRI1参与了种子灌浆过程中碳水化合物代谢的发育调控。
During oil deposition in developing seeds of Arabidopsis, photosynthate is imported in the form of carbohydrates into the embryo and converted to triacylglycerols. To identify genes essential for this process and to investigate the molecular basis for the developmental regulation of oil accumulation, mutants producing wrinkled, incompletely filled seeds were isolated. A novel mutant locus, wrinkled1 (wri1), which maps to the bottom of chromosome 3 and causes an 80% reduction in seed oil content, was identified. Wildtype and homozygous wri1 mutant plantlets or mature plants were indistinguishable. However, developing homozygous wri1 seeds were impaired in the incorporation of sucrose and glucose into triacylglycerols, but incorporated pyruvate and acetate at an increased rate. Because the activities of several glycolytic enzymes, in particular hexokinase and pyrophosphate-dependent phosphofructokinase, are reduced in developing homozygous wri1 seeds, it is suggested that WRI1 is involved in the developmental regulation of carbohydrate metabolism during seed filling.