Influence of clinical factors, diet, and drugs on the human upper gastrointestinal glutathione system
Influence of clinical factors, diet, and drugs on the human upper gastrointestinal glutathione system
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DOI：
10.1136/gut.50.2.235
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发表时间：
2002-02-01
期刊：
影响因子：
24.5
通讯作者：
Kirch, W
中科院分区：
文献类型：
--
作者：
Hoensch, H;Morgenstern, I;Kirch, W
背景：谷胱甘肽(GSH)和胞内谷胱甘肽S转移酶(GSTs)保护胃肠粘膜免受多种化合物的毒性作用，如活性氧和亲电体。目的：研究上消化道黏膜的分布和临床变量对GSH系统组成的影响，以进一步了解控制其细胞保护作用的因素。方法：收集2 0 2例上消化道胃镜检查患者的胃窦和十二指肠活检标本，男10 4例，女98例，平均年龄62岁。结果：GSH、GST酶活性和GSTα水平显著降低(P<0.05)。
Background: Glutathione (GSH) and the cytosolic glutathione S-transferases (GSTs) protect the gastrointestinal mucosa against the toxic effects of a wide variety of compounds, such as reactive oxygen species and electrophiles.Aims: We wished to investigate the distribution along the upper gastrointestinal mucosa and the influence of clinical variables on components of the GSH system to learn more about factors which control its cytoprotective properties.Methods: Antral and duodenal biopsies of normal appearing mucosa were collected from 202 patients (104 males, 98 females; mean age 62 years) undergoing upper gastrointestinal endoscopy. GSH content was examined by high pressure liquid chromatography, GST enzyme activity by 1-chloro-, 2, 4-dinitrobenzene conjugation, and levels of the GST classes alpha, pi, and theta by western blot.Results: GSH, GST enzyme activity, and GST alpha levels were significantly lower (p