Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation
Identification of cardiac stem cells with FLK1, CD31, and VE-cadherin expression during embryonic stem cell differentiation
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DOI：
10.1096/fj.04-1998com
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发表时间：
2005-03-01
期刊：
影响因子：
4.8
通讯作者：
Nakahata, T
中科院分区：
文献类型：
--
作者：
Iida, M;Heike, T;Nakahata, T
我们评估了FLK 1的表达，FLK 1是发生心脏发生的外侧中胚层早期标志物之一，以表征和分离ES细胞中的心脏干/祖细胞。在第3、4或5天从胚状体（EB）分离的细胞被收集到具有或不具有FLK 1表达的两个亚群中，并继续在OP 9基质细胞上共培养以检查收缩集落是否出来。第3天和第4天来自EB的FLK 1（+）细胞比同一天的FLK 1（-）细胞更有效地形成自发收缩集落，但在第5天不是。主要在第4天在内皮细胞上检测到来自FLK 1细胞的大多数收缩的心脏集落，沿着造血细胞。将具有这些能力的细胞进一步表征为三个谱系，揭示了FLK 1（+）CD 31-VE-钙粘蛋白表型。我们的研究结果表明，FLK 1（+）细胞，特别是FLK 1（+）CD 31（-）VE-钙粘素（-）细胞，可以作为心脏成血管细胞，形成心脏细胞以及内皮细胞和造血细胞。
We evaluated the expression of the FLK1, one of the lateral mesoderim early markers where cardiogenesis occurs, to characterize and isolate cardiac stem/progenitor cells from ES cells. Dissociated cells from embryoid bodies (EBs) on day 3, 4, or 5 were collected into two subpopulations with or without FLK1 expression and coculture on OP9 stromal cells was continued to examine whether contracting colonies came out or not. FLK1(+) cells from EBs at days 3 and 4 formed spontaneous contracting colonies more efficiently than FLK1(-) cells on the same days, but not at day 5. Most contracting cardiac colonies derived from FLK1 cells mainly on day 4 were detected on endothelial cells along with hematopoietic cells. Further characterization of cells with these capabilities into three lineages revealed the FLK1(+) CD31-VE-cadherin phenotype. Our findings indicate that FLK1(+) cells, especially FLK1(+) CD31(-)VE-cadherin(-) cells, could act as cardiohemangioblasts to form cardiac cells as well as endothelial cells and hematopoietic cells.