DNA stretching on functionalized gold surfaces.
DNA stretching on functionalized gold surfaces.
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DOI：
10.1093/nar/22.3.492
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发表时间：
1994-02
影响因子：
14.9
通讯作者：
Ralf M. Zimmermann;Edward C. Cox
中科院分区：
文献类型：
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作者：
Ralf M. Zimmermann;Edward C. Cox
我们描述了一种通过金-生物素-链霉亲和素-生物素-DNA键将噬菌体lambda DNA一端锚定在金上的方法。锚定在微制金线上的DNA可以在流场和电场中排列和拉伸。锚定被证明可以抵抗至少11pN的力，这种连接足够强大，足以使染色体大小的DNA分子被拉伸和排列。
We describe a method for anchoring bacteriophage lambda DNA by one end to gold by Au-biotin-streptavidin-biotin-DNA bonds. DNA anchored to a microfabricated Au line could be aligned and stretched in flow and electric fields. The anchor was shown to resist a force of at least 11 pN, a linkage strong enough to allow DNA molecules of chromosome size to be stretched and aligned.