Natural Products Diversity in Plant-Insect Interaction between Tithonia diversifolia (Asteraceae) and Chlosyne lacinia (Nymphalidae)
Natural Products Diversity in Plant-Insect Interaction between Tithonia diversifolia (Asteraceae) and Chlosyne lacinia (Nymphalidae)
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DOI：
10.3390/molecules24173118
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发表时间：
2019-09-01
期刊：
影响因子：
4.6
通讯作者：
Gobbo-Neto, Leonardo
中科院分区：
文献类型：
--
作者：
Gallon, Marilia Elias;Silva-Junior, Eduardo Afonso;Gobbo-Neto, Leonardo
植物-昆虫相互作用的化学生态学已经推动我们对生态系统进化的理解进入一个更全面的背景。小食心虫（Chlosyne lacinia）是鳞翅目（Lessoptera）睡莲科（Nymphalidae）的一种多食性草食性昆虫，主要以菊科（Asteraceae）向日葵族（Heliantheae）植物为寄主。本文采用非靶向LC-MS/MS代谢组学和分子网络技术，研究了多叶铁炮花（Tithonia diversifolia，Heliantheae）与小绿叶蝉（Chlosyne lacinia）之间的植物-昆虫相互作用，旨在探索其内在的化学多样性。C.用T. diversifolia叶发育至5龄，完成变态至成虫期。在T. diversifolia（叶和非消耗背面）和C. lacinia（粪便、幼虫、蛹、蝴蝶和卵）样品。我们发现在T. diversifolia以完整形态被C.条格孢幼虫因此，C.多年来，lacinia毛虫可能已经在其消化道的整个有效屏障中形成了对STL的耐受机制。黄苷元主要存在于T.而其苷类成分主要在胡芦巴中检出。lacinia feces，表明黄酮类化合物的糖基化是其排泄的主要机制。此外，溶血磷脂主要存在于C。lacinia样品中的代谢产物，表明它们是蛹期和成虫期的必需代谢产物。这些发现提供了深入了解这种植物-昆虫相互作用的天然产物多样性，并有助于揭示其生态作用。
The chemical ecology of plant-insect interactions has been driving our understanding of ecosystem evolution into a more comprehensive context. Chlosyne lacinia (Lepidoptera: Nymphalidae) is an olygophagous insect herbivore, which mainly uses host plants of Heliantheae tribe (Asteraceae). Herein, plant-insect interaction between Tithonia diversifolia (Heliantheae) and Chlosyne lacinia was investigated by means of untargeted LC-MS/MS based metabolomics and molecular networking, which aims to explore its inherent chemical diversity. C. lacinia larvae that were fed with T. diversifolia leaves developed until fifth instar and completed metamorphosis to the adult phase. Sesquiterpene lactones (STL), flavonoids, and lipid derivatives were putatively annotated in T. diversifolia (leaves and non-consumed abaxial surface) and C. lacinia (feces, larvae, pupae, butterflies, and eggs) samples. We found that several furanoheliangolide-type STL that were detected in T. diversifolia were ingested and excreted in their intact form by C. lacinia larvae. Hence, C. lacinia caterpillars may have, over the years, developed tolerance mechanisms for STL throughout effective barriers in their digestive canal. Flavonoid aglycones were mainly found in T. diversifolia samples, while their glycosides were mostly detected in C. lacinia feces, which indicated that the main mechanism for excreting the consumed flavonoids was through their glycosylation. Moreover, lysophospholipids were predominately found in C. lacinia samples, which suggested that they were essential metabolites during pupal and adult stages. These findings provide insights into the natural products diversity of this plant-insect interaction and contribute to uncovering its ecological roles.