Phase behavior of isolated photoreceptor membrane lipids is modulated by bivalent cations
Phase behavior of isolated photoreceptor membrane lipids is modulated by bivalent cations
复制标题
分离的光感受器膜脂的相行为由二价阳离子调节
DOI：
10.1016/0014-5793(84)80329-4
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发表时间：
1984
期刊：
影响因子：
3.5
通讯作者：
W. D. de Grip
中科院分区：
文献类型：
--
作者：
L. Mollevanger;W. D. de Grip
鉴于先前不一致的结果[(1979)Biochim]，用31P-核磁共振进一步研究了分离的光感受器膜脂的相行为。生物群落。学报558,330-337；(1982)FEBS Lett。124、93-99]。我们提出的证据表明，这种差异是由于二价阳离子造成的。在无二价阳离子存在的中性条件下，再悬浮于水介质中时，分离的光感受器膜脂主要采用双层构型。然而，当加入这种阳离子(钙镁离子)或在它们存在的情况下重新悬浮时，会导致其他相的形成(六方HII，脂类颗粒)。这种转变的速度取决于阳离子浓度和温度。这种转变不容易被EDTA的加入所逆转。关于光感受器膜结构和功能的影响需要进一步研究。
The phase behavior of isolated photoreceptor membrane lipids is further investigated by31P‐NMR, in view of earlier discrepant results [(1979) Biochim. Biophys. Acta 558, 330–337; (1982) FEBS Lett. 124, 93–99]. We present evidence that the discrepancy is due to bivalent cations. When resuspended in aqueous media at neutral pH in the absence of bivalent cations, the isolated photoreceptor membrane lipids largely adopt the bilayer configuration. However, upon addition of such cations (Ca2+Mg2+) or when resuspended in their presence, the formation of other phases (hexagonal HII, lipidic particles) results. The rate of this transition depends on cation concentration and temperature. The transition is not easily reversed by addition of EDTA. Implications with regard to photoreceptor membrane structure and function need further study.