Adrenal adrenaline- and noradrenaline-containing cells and celiac sympathetic ganglia are differentially controlled by centrally administered corticotropin-releasing factor and arginine-vasopressin in rats
Adrenal adrenaline- and noradrenaline-containing cells and celiac sympathetic ganglia are differentially controlled by centrally administered corticotropin-releasing factor and arginine-vasopressin in rats
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DOI：
10.1016/j.ejphar.2007.02.021
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发表时间：
2007-06-14
影响因子：
5
通讯作者：
Yokotani, Kunihiko
中科院分区：
文献类型：
--
作者：
Yamaguchi-Shima, Naoko;Okada, Shoshiro;Yokotani, Kunihiko
肾上腺和腹腔交感神经节主要由内脏大神经支配，内脏大神经包含起源于胸脊髓的节前交感神经。肾上腺髓质有两个独立的群体的嗜钟细胞，肾上腺素含细胞（A细胞）和去甲肾上腺素含细胞（NA细胞），已被证明是不同的神经支配的不同群体的节前交感神经元。本研究旨在描述麻醉大鼠肾上腺A细胞、NA细胞和腹腔交感神经节表达cFos（神经兴奋的标志物）对脑前列腺素的中枢激活机制。侧脑室注射（i. c. v.）给予促肾上腺皮质激素释放因子（CRF）诱导肾上腺A细胞（但不诱导NA细胞）和腹腔神经节中的cFos表达。另一方面，i. c. v.给予精氨酸加压素（AVP）导致肾上腺髓质中A细胞和NA细胞中的cFos诱导，但在腹腔神经节中没有。侧脑室注射吲哚美辛（一种环氧合酶抑制剂）可阻断CRF和AVP诱导的上述所有区域cFos表达。另一方面，侧脑室注射furegrelate（血栓素A2合酶抑制剂）可抑制CRF诱导的肾上腺A细胞cFos表达，但不能抑制腹腔神经节cFos表达，同时也可抑制ANT诱导的肾上腺髓质A细胞和NA细胞cFos表达。这些结果表明，中央给药的CRF分别通过脑血栓素A（2）介导的机制和血栓素A（2）以外的其他前列腺素（可能是前列腺素E-2）介导的机制激活肾上腺A细胞和腹腔交感神经节。另一方面，在大鼠中，中枢给予AVP通过脑血栓素A（2）介导的机制激活肾上腺A细胞和NA细胞。(c)2007 Elsevier B. V.保留所有权利。
The adrenal glands and sympathetic celiac ganglia are innervated mainly by the greater splanchnic nerves, which contain preganglionic sympathetic nerves that originated from the thoracic spinal cord. The adrenal medulla has two separate populations of chrontaffin cells, adrenalinecontaining cells (A-cells) and noradrenaline-containing cells (NA-cells), which have been shown to be differentially innervated by separate groups of the preganglionic sympathetic neurons. The present study was designed to characterize the centrally activating mechanisms of the adrenal A-cells, NA-cells and celiac sympathetic ganglia with expression of cFos (a marker for neural excitation), in regard to the brain prostanoids, in anesthetized rats. Intracerebroventricularly (i.c.v.) administered corticotropin-releasing factor (CRF) induced cFos expression in the adrenal A-cells, but not NA-cells, and celiac ganglia. On the other hand, i.c.v. administered arginine-vasopressin (AVP) resulted in cFos induction in both A-cells and NA-cells in the adrenal medulla, but not in the celiac ganglia. Intracerebroventricular pretreatment with indomethacin (an inhibitor of cyclooxygenase) abolished the CRF- and AVP-induced cFos expression in all regions described above. On the other hand, intracerebroventricular pretreatment with furegrelate (an inhibitor of thromboxane A(2) synthase) abolished the CRF-induced cFos expression in the adrenal A-cells, but not in the celiac ganglia, and also abolished the ANT-induced cFos expression in both A-cells and NA-cells in the adrenal medulla. These results suggest that centrally administered CRF activates adrenal A-cells and celiac sympathetic ganglia by brain thromboxane A(2)-mediated and other prostanoid than thromboxane A(2) (probably prostaglandin E-2)-mediated mechanisms, respectively. On the other hand, centrally administered AVP activates adrenal A-cells and NA-cells by brain thromboxane A(2)-mediated mechanisms in rats. (c) 2007 Elsevier B.V. All rights reserved.