Circulating micrometastases of esophageal cancer detected by carcinoembryonic antigen mRNA reverse transcriptase-polymerase chain reaction: clinical implications
Circulating micrometastases of esophageal cancer detected by carcinoembryonic antigen mRNA reverse transcriptase-polymerase chain reaction: clinical implications
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DOI：
10.1111/j.1442-2050.2008.00830.x
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发表时间：
2008-01-01
影响因子：
2.6
通讯作者：
Tsurumaru, M.
中科院分区：
文献类型：
--
作者：
Hashimoto, T.;Kajiyama, Y.;Tsurumaru, M.
根据传统的术前检查，在一些没有远处转移的患者中，食道癌根治性切除后几年内远处器官就会复发。已经进行了各种尝试来检测常规技术无法发现的微转移。采用实时定量逆转录聚合酶链式反应(RT-PCR)检测2003年9月至2004年6月在君田大学医院接受食道癌根治术的49例患者的147份血样中癌胚抗原的信使RNA。评估了循环癌细胞的数量，并分析了检测此类微转移的临床意义。多因素分析显示，Pt1或Pt2阳性与Pt1或Pt2疾病、III或IV期疾病显著相关。定量分析10(4)个正常细胞中癌胚抗原基因拷贝数在40~50个以上的患者复发率较高。与肿瘤细胞的存在相比，血液中循环的肿瘤细胞数量对食道癌预后的影响可能更大。
In some patients without distant metastases according to conventional preoperative investigations, relapse occurs in distant organs within a few years after radical resection of esophageal cancer. Various attempts have been made to detect micrometastases that are not found by conventional techniques. A quantitative real-time reverse-transcriptase polymerase chain reaction was used to detect messenger RNA for carcinoembryonic antigen in 147 blood samples from 49 patients scheduled for radical resection of esophageal cancer at Juntendo University Hospital between September 2003 and June 2004. The number of circulating cancer cells was assessed and the clinical significance of detecting such micrometastases was analyzed. Multivariate analysis showed that positivity of this assay was significantly associated with pT1 or pT2 disease and stage III or stage IV disease. Patients with more than 40-50 carcinoembryonic antigen mRNA copies among 10(4) normal cells on quantitative analysis had a higher recurrence rate. The number of tumor cells circulating in the blood may have more influence on the prognosis of esophageal cancer than the presence of tumor cells.