The association of nuclear blebs with aneuploidy in human acute leukemia.
The association of nuclear blebs with aneuploidy in human acute leukemia.
复制标题
核泡与人类急性白血病非整倍性的关联。
作者：
M. Ahearn;J. Trujillo;A. Cork;A. Fowler;J. Hart
在我们实验室，监测处于疾病活动期和缓解期的急性白血病患者的骨髓研究表明，非整倍体与一种特定的超微结构细胞异常(核泡)之间存在明显的关联。为了确定这种相关性的程度，对急性白血病患者的骨髓标本进行了同步的细胞遗传学和超微结构分析。作为本研究的补充参数，对20例急性白血病患者和6例正常对照的骨髓标本进行了超微结构定量分析。这些相关研究的结果表明，在疾病的活动期，非整倍体与高频率的核泡有一定的相关性。成功的治疗反应伴随着染色体和超微结构异常细胞的显着减少或完全消失。相反，在这些个体中，两种核异常的再现预示着即将复发。这些细胞学改变可能为评估患者对治疗的反应提供有用的工具。
Summary In our laboratory, bone marrow studies that monitored acute leukemia patients in the active phases of the disease and in the remission state demonstrated an apparent association between aneuploidy and a specific ultrastructural cellular abnormality (nuclear blebs). In order to determine the extent of this correlation, bone marrow specimens from patients with acute leukemia were investigated by simultaneous cytogenetic and ultrastructural analyses. As an additional parameter of this study, a quantitative ultrastructural analysis was carried out in bone marrow samples from 20 of the acute leukemia patients, as well as from 6 normal controls. Results from these correlated studies show that, during the active phase of the disease, there is a definite correlation between aneuploidy and a high frequency of nuclear blebs. Successful response to therapy is accompanied by a marked reduction or total disappearance of the chromosomally and ultrastructurally abnormal cells. Conversely, impending relapse in these individuals is indicated by the reappearance of both nuclear abnormalities. These cytological alterations may provide a useful tool for evaluating patient response to therapy.