Internal dialysis of Limulus ventral photoreceptors.
Internal dialysis of Limulus ventral photoreceptors.
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鲎腹侧光感受器的内部透析。
DOI：
10.1073/pnas.79.23.7580
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发表时间：
1982
影响因子：
11.1
通讯作者：
Lisman,JE
中科院分区：
文献类型：
--
作者：
Stern,JH;Lisman,JE
内透析技术已应用于鲎腹侧光感受器。这种方法有可能对活细胞内可扩散分子的浓度进行定量控制。在透析过程中，鲎光感受器保留了对光的反应能力。在昏暗的照明条件下，反应在近一个小时内是正常的。在明亮的光线下，异常发展得更快。提高透析液Mg2+浓度和罗丹明标记白蛋白进入细胞的可逆效应表明，透析方法可用于分析小分子或大分子对视觉转导的影响。这种方法已被用于检验三磷酸核苷酸和环核苷酸的影响。结果表明，三磷酸核苷酸（5-10 mM）可以维持较低的自发量子颠簸率。环核苷酸在转导中的重要性尚不清楚；cGMP或cAMP均可降低光响应，但仅在非常高浓度（10 mM）时。
The internal dialysis technique has been applied to Limulus ventral photoreceptors. This method potentially allows quantitative control of the concentration of diffusible molecules within living cells. During dialysis, Limulus photoreceptors retained their ability to respond to light. Under conditions of dim illumination, responses were normal for close to an hour. In bright light, abnormalities developed more rapidly. The reversible effects of raising the dialysate Mg2+ concentration and the entrance of rhodamine-labeled albumin into cells shows that the dialysis method is useful for assaying the effects of small or large molecules on visual transduction. This method has been used to examine the effects of nucleotide triphosphates and cyclic nucleotides. The results show that nucleotide triphosphates (5-10 mM) are required to maintain a low rate of spontaneous quantum bumps. The importance of cyclic nucleotides in transduction is less clear; the light response was reduced by either cGMP or cAMP but only at very high concentrations (10 mM).